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Cell Sorting, Culture, Preconditioning, and Modulation/Cell Aggregates: Sheets

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Vascularization for Tissue Engineering and Regenerative Medicine

Part of the book series: Reference Series in Biomedical Engineering ((TIENRE))

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Abstract

A novel cell culture surface grafted with the temperature-responsive polymer poly(N-isopropylacrylamide) has been developed for controlled attachment and detachment of living cells through temperature changes. This temperature-responsive surface allows us to harvest confluent cells in the form of an intact monolayer cell sheet with deposited extra cellular matrices. Different from scaffold-based tissue engineering, cell sheet-based tissue engineering without the use of scaffolds provides another way for the development of tissue engineering. Taking advantage of the unique ability of cell sheets to generate three-dimensional (3D) tissues, we have also developed several methods to stack cell sheets and create functional tissues for therapy of a vast variety of diseases ranging from periodontitis and corneal disease to esophageal ulceration and heart failure. Moreover, we have created functional anisotropic tissues, thick vascularized tissues, and even organ-like systems by using cell sheet-based tissue engineering. In this chapter, we summarize development of temperature-responsive cell culture surfaces, cell sheet manipulation technologies, fabrication of thick tissues, and organ-like structures with cell sheets as well as applications of cell sheet-based tissue engineering.

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Authors and Affiliations

  1. Institute of Advanced Biomedical Engineering and Science, Tokyo Women’s Medical University, Shinjuku-ku, Tokyo, Japan

    Botao Gao, Tatsuya Shimizu & Teruo Okano

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  1. Botao Gao
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  2. Tatsuya Shimizu
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  3. Teruo Okano
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Corresponding author

Correspondence to Teruo Okano .

Editor information

Editors and Affiliations

  1. Austrian Cluster for Tissue Engineering, Vienna, Austria

    Wolfgang Holnthoner

  2. Department of Biomedicine, University Hospital Basel, Basel, Switzerland

    Andrea Banfi

  3. Institute for Pathology, Johannes Gutenberg University, Mainz, Germany

    James Kirkpatrick

  4. Ludwig Boltzmann Institute for Experimental and Clinical Traumatology, AUVA Research Center, Austrian Cluster for Tissue Regeneration, Wien, Austria

    Heinz Redl

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Gao, B., Shimizu, T., Okano, T. (2021). Cell Sorting, Culture, Preconditioning, and Modulation/Cell Aggregates: Sheets. In: Holnthoner, W., Banfi, A., Kirkpatrick, J., Redl, H. (eds) Vascularization for Tissue Engineering and Regenerative Medicine. Reference Series in Biomedical Engineering(). Springer, Cham. https://doi.org/10.1007/978-3-319-54586-8_18

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