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Analysis of Endogenous Metabolites in Human Matrices

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Handbook of Bioanalytics

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Abstract

For several decades, biological matrices have been used to assess the physiological conditions of organisms. The development of analytical techniques and the introduction of more sensitive and accurate methods have enabled to detect endogenous metabolites present in biological matrices and their effect on homeostasis maintenance. Consequently, it has broadened the scope of application of biological matrices in bioanalysis. Biological matrices are used to search new bioindicators of the occurrence and development of different diseases, to assess the body’s response to therapy, and to retrospectively evaluate metabolic changes in the organism over a long period of time, which is particularly useful in toxicology and crime detection methods. The detection of endogenous compounds in biological matrices is performed in the field of -omics sciences such as metabolomics, which involves the study of low-molecular-weight compounds and products of biochemical transformations, i.e., metabolites. Two research strategies are used in metabolomics, namely, targeted and nontargeted metabolomic approach, depending on whether the study is based on the qualitative determination of the widest possible panel of compounds or is a narrow quantitative one restricted to previously selected metabolites. Currently, endogenous metabolites are studied in various biological matrices. This chapter describes the most commonly used human matrices such as blood, urine, and tissues and less common ones (saliva and exhaled breath) as well as unconventional matrices such as hair, nails, tears, sweat, cerebrospinal fluid, ejaculate, and aqueous humor.

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Author information

Authors and Affiliations

  1. Department of Biopharmaceutics and Pharmacodynamics, Faculty of Pharmacy, Medical University of Gdańsk, Gdańsk, Poland

    Wiktoria Struck-Lewicka, Szymon Macioszek, Małgorzata Artymowicz, Małgorzata Waszczuk-Jankowska, Danuta Siluk & Michał J. Markuszewski

Authors
  1. Wiktoria Struck-Lewicka
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  2. Szymon Macioszek
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  3. Małgorzata Artymowicz
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  4. Małgorzata Waszczuk-Jankowska
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  5. Danuta Siluk
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  6. Michał J. Markuszewski
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Corresponding author

Correspondence to Michał J. Markuszewski .

Editor information

Editors and Affiliations

  1. Environmental Chemistry and Bioanalytics, Nicolaus Copernicus University, Torun, Poland

    Bogusław Buszewski

  2. Department of Inorganic Chemistry, Analytical Chemistry and Electrochemistry, Faculty of Chemistry, Silesian University of Technology, Gliwice, Poland

    Irena Baranowska

Section Editor information

  1. Centre National de la Recherche Scientifique (CNRS), Institute of Analytical Sciences and Physico-Chemistry for Environment (IPREM, UMR 5254 CNRS-UPPA), Pau, France

    Joanna Szpunar

  2. Faculty of Chemistry, Warsaw University of Technology, Warsaw, Poland

    Ryszard Łobiński

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Struck-Lewicka, W., Macioszek, S., Artymowicz, M., Waszczuk-Jankowska, M., Siluk, D., Markuszewski, M.J. (2022). Analysis of Endogenous Metabolites in Human Matrices. In: Buszewski, B., Baranowska, I. (eds) Handbook of Bioanalytics. Springer, Cham. https://doi.org/10.1007/978-3-030-95660-8_4

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