Marine-Derived Hydroxyapatite for Tissue Engineering Strategies

Anil, Sukumaran; Sweety, Vishnupriya K.; Joseph, Betsy

doi:10.1007/978-3-030-92090-6_60-1

Sukumaran Anil^4,5,
Vishnupriya K. Sweety⁶ &
Betsy Joseph^6,7

25 Accesses

Abstract

Marine hydroxyapatite is a naturally occurring mineral present in the skeletal structures of various marine organisms, including fish bones, shells, and corals. This biogenic form of hydroxyapatite, represented by the chemical formula Ca₁₀(PO₄)₆(OH)₂, has given rise to marine-derived hydroxyapatite (MHA), a biomaterial that shows immense promise for tissue engineering applications due to its biocompatibility, bioactivity, and osteoconductivity. This chapter offers a comprehensive overview of the sources, chemical characterization, and distinct properties of MHA, emphasizing its potential benefits over synthetic hydroxyapatite within the scope of tissue engineering. Furthermore, the chapter delves into the existing and prospective applications of MHA in an array of tissue engineering strategies. By shedding light on the current challenges and future research avenues for the development and optimization of MHA-based tissue engineering strategies, the chapter underscores the need for interdisciplinary cooperation and pioneering approaches to fully harness the extraordinary potential of this marine-derived biomaterial.

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References

Ağaoğulları D, Kel D, Gökçe H, Duman I, Öveçoğlu ML, Akarsubaşı AT, Bilgiç D, Oktar FN. Bioceramic production from sea urchins. Acta Phys Pol A. 2012;121:23–5.
Article Google Scholar
Ahamed AF, Manimohan M, Kalaivasan N. Fabrication of biologically active fish bone derived hydroxyapatite and montmorillonite blended sodium alginate composite for in-vitro drug delivery studies. J Inorg Organomet Polym Mater. 2022;32(10):3902–22. https://doi.org/10.1007/s10904-022-02401-1.
Article CAS Google Scholar
Akram M, Ahmed R, Shakir I, Ibrahim WAW, Hussain R. Extracting hydroxyapatite and its precursors from natural resources. J Mater Sci. 2013;49(4):1461–75. https://doi.org/10.1007/s10853-013-7864-x.
Article CAS Google Scholar
Aminatun SA, Nisa ZI, Hikmawati D, Siswanto. Synthesis of nanohydroxyapatite from cuttlefish bone (Sepia sp.) using milling method. Int J Biomater. 2019;2019:1831208. https://doi.org/10.1155/2019/1831208.
Article CAS PubMed PubMed Central Google Scholar
Anjum S, Rahman F, Pandey P, Arya DK, Alam M, Rajinikanth PS, Ao Q. Electrospun biomimetic nanofibrous scaffolds: a promising prospect for bone tissue engineering and regenerative medicine. Int J Mol Sci. 2022;23(16):9206. https://doi.org/10.3390/ijms23169206.
Article CAS PubMed PubMed Central Google Scholar
Ashwitha A, Thamizharasan K, Bhatt P. Optimization of hydroxyapatite (HAp) extraction from scales of Sardinella longiceps and its conjugative effect with immunostimulants. SN Appl Sci. 2020;2(7):1228. https://doi.org/10.1007/s42452-020-3057-9.
Article CAS Google Scholar
Bernhardt A, Paul B, Gelinsky M. Biphasic scaffolds from marine collagens for regeneration of osteochondral defects. Mar Drugs. 2018;16(3):91. https://doi.org/10.3390/md16030091.
Article CAS PubMed PubMed Central Google Scholar
Bilić-Prcić M, Šalinović I, Gurgan S, Koc Vural U, Krmek SJ, Miletić I. Effects of incorporation of marine derived hydroxyapatite on the microhardness, surface roughness, and fluoride release of two glass-Ionomer cements. Appl Sci. 2021;11(22):11027.
Article Google Scholar
Cestari F, Petretta M, Yang Y, Motta A, Grigolo B, Sglavo VM. 3D printing of PCL/nano-hydroxyapatite scaffolds derived from biogenic sources for bone tissue engineering. Sustain Mater Technol. 2021;29:e00318. https://doi.org/10.1016/j.susmat.2021.e00318.
Article CAS Google Scholar
Chen J. Recent development of biomaterials combined with mesenchymal stem cells as a strategy in cartilage regeneration. Int J Transl Med. 2022;2(3):456–81.
Google Scholar
Chen S, Shi Y, Zhang X, Ma J. Evaluation of BMP-2 and VEGF loaded 3D printed hydroxyapatite composite scaffolds with enhanced osteogenic capacity in vitro and in vivo. Mater Sci Eng C Mater Biol Appl. 2020;112:110893. https://doi.org/10.1016/j.msec.2020.110893.
Article CAS PubMed Google Scholar
Clarke SA, Choi SY, McKechnie M, Burke G, Dunne N, Walker G, Cunningham E, Buchanan F. Osteogenic cell response to 3-D hydroxyapatite scaffolds developed via replication of natural marine sponges. J Mater Sci Mater Med. 2016;27(2):22. https://doi.org/10.1007/s10856-015-5630-0.
Article CAS PubMed Google Scholar
Dai M, Sui B, Xue Y, Liu X, Sun J. Cartilage repair in degenerative osteoarthritis mediated by squid type II collagen via immunomodulating activation of M2 macrophages, inhibiting apoptosis and hypertrophy of chondrocytes. Biomaterials. 2018;180:91–103. https://doi.org/10.1016/j.biomaterials.2018.07.011.
Article CAS PubMed Google Scholar
Deb P, Deoghare AB. Effect of acid, alkali and alkali–acid treatment on physicochemical and bioactive properties of hydroxyapatite derived from catla catla fish scales. Arab J Sci Eng. 2019;44(9):7479–90.
Article CAS Google Scholar
Desmond S, Kurniawan D, Nor FM. Synthesis of hydroxyapatite from seashells via calcination at various temperature using microwave and furnace. AIP Conf Proc. 2020;2217(1):020010. https://doi.org/10.1063/5.0000902.
Article CAS Google Scholar
DiCarlo BB, Hu JC, Gross T, Vago R, Athanasiou KA. Biomaterial effects in articular cartilage tissue engineering using polyglycolic acid, a novel marine origin biomaterial, IGF-I, and TGF-beta 1. Proc Inst Mech Eng H. 2009;223(1):63–73. https://doi.org/10.1243/09544119JEIM424.
Article CAS PubMed Google Scholar
Dorozhkin SV. Bioceramics of calcium orthophosphates. Biomaterials. 2010;31(7):1465–85. https://doi.org/10.1016/j.biomaterials.2009.11.050.
Article CAS PubMed Google Scholar
Filip DG, Surdu V-A, Paduraru AV, Andronescu E. Current development in biomaterials—hydroxyapatite and bioglass for applications in biomedical field: a review. J Funct Biomater. 2022;13(4):248.
Article CAS PubMed PubMed Central Google Scholar
Firdaus Hussin MS, Abdullah HZ, Idris MI, Abdul Wahap MA. Extraction of natural hydroxyapatite for biomedical applications-a review. Heliyon. 2022;8(8):e10356. https://doi.org/10.1016/j.heliyon.2022.e10356.
Article CAS PubMed PubMed Central Google Scholar
Gao W, Chen Y, Zhang Y, Zhang Q, Zhang L. Nanoparticle-based local antimicrobial drug delivery. Adv Drug Deliv Rev. 2018;127:46–57. https://doi.org/10.1016/j.addr.2017.09.015.
Article CAS PubMed Google Scholar
Gille J, Dorn B, Kekow J, Bruns J, Behrens P. Bone substitutes as carriers for transforming growth factor-beta(1) (TGF-beta(1)). Int Orthop. 2002;26(4):203–6. https://doi.org/10.1007/s00264-002-0353-5.
Article CAS PubMed PubMed Central Google Scholar
Granito RN, Muniz Renno AC, Yamamura H, de Almeida MC, Menin Ruiz PL, Ribeiro DA. Hydroxyapatite from fish for bone tissue engineering: a promising approach. Int J Mol Cell Med. 2018;7(2):80–90. https://doi.org/10.22088/IJMCM.BUMS.7.2.80.
Article CAS PubMed PubMed Central Google Scholar
Gugliandolo A, Fonticoli L, Trubiani O, Rajan TS, Marconi GD, Bramanti P, Mazzon E, Pizzicannella J, Diomede F. Oral bone tissue regeneration: Mesenchymal stem cells, secretome, and biomaterials. Int J Mol Sci. 2021;22(10):5236. https://doi.org/10.3390/ijms22105236.
Article CAS PubMed PubMed Central Google Scholar
Huang Y-C, Hsiao P-C, Chai H-J. Hydroxyapatite extracted from fish scale: effects on MG63 osteoblast-like cells. Ceram Int. 2011;37(6):1825–31. https://doi.org/10.1016/j.ceramint.2011.01.018.
Article CAS Google Scholar
Ikada Y. Challenges in tissue engineering. J R Soc Interface. 2006;3(10):589–601. https://doi.org/10.1098/rsif.2006.0124.
Article CAS PubMed PubMed Central Google Scholar
Jindapon N, Klinmalai P, Surayot U, Tanadchangsaeng N, Pichaiaukrit W, Phimolsiripol Y, Vichasilp C, Wangtueai S. Preparation, characterization, and biological properties of hydroxyapatite from bigeye snapper (Priancanthus tayenus) bone. Int J Mol Sci. 2023;24(3):2776. https://doi.org/10.3390/ijms24032776.
Article CAS PubMed PubMed Central Google Scholar
Khojasteh A, Motamedian SR, Rad MR, Shahriari MH, Nadjmi N. Polymeric vs hydroxyapatite-based scaffolds on dental pulp stem cell proliferation and differentiation. World J Stem Cells. 2015;7(10):1215–21. https://doi.org/10.4252/wjsc.v7.i10.1215.
Article PubMed PubMed Central Google Scholar
Komur B, Altun E, Aydogdu M, Bilgiç D, Gokce H, Ekren N, Salman S, Inan A, Oktar F, Gunduz O. Hydroxyapatite synthesis from fish bones: Atlantic salmon (Salmon salar). Acta Phys Polon. 2017;131(3):400–2.
Article CAS Google Scholar
LeGeros RZ. Properties of osteoconductive biomaterials: calcium phosphates. Clin Orthop Relat Res. 2002;395:81–98. https://doi.org/10.1097/00003086-200202000-00009.
Article Google Scholar
Liao W, Xu L, Wangrao K, Du Y, Xiong Q, Yao Y. Three-dimensional printing with biomaterials in craniofacial and dental tissue engineering. PeerJ. 2019;7:e7271. https://doi.org/10.7717/peerj.7271.
Article PubMed PubMed Central Google Scholar
Matta C, Szucs-Somogyi C, Kon E, Robinson D, Neufeld T, Altschuler N, Berta A, Hangody L, Vereb Z, Zakany R. Osteogenic differentiation of human bone marrow-derived mesenchymal stem cells is enhanced by an aragonite scaffold. Differentiation. 2019;107:24–34. https://doi.org/10.1016/j.diff.2019.05.002.
Article CAS PubMed Google Scholar
Mehnath S, Arjama M, Rajan M, Premkumar K, Karthikeyan K, Jeyaraj M. Mineralization of bioactive marine sponge and electrophoretic deposition on Ti-6Al-4V implant for osteointegration. Surf Coat Technol. 2020;392:125727. https://doi.org/10.1016/j.surfcoat.2020.125727.
Article CAS Google Scholar
Mitran V, Ion R, Miculescu F, Necula MG, Mocanu AC, Stan GE, Antoniac IV, Cimpean A. Osteoblast cell response to naturally derived calcium phosphate-based materials. Materials (Basel). 2018;11(7):1097. https://doi.org/10.3390/ma11071097.
Article CAS PubMed Google Scholar
Mohan S, Raghavendran HB, Karunanithi P, Murali MR, Naveen SV, Talebian S, Mehrali M, Mehrali M, Natarajan E, Chan CK, Kamarul T. Incorporation of human-platelet-derived growth factor-BB encapsulated poly(lactic-co-glycolic acid) microspheres into 3D CORAGRAF enhances osteogenic differentiation of mesenchymal stromal cells. ACS Appl Mater Interfaces. 2017;9(11):9291–303. https://doi.org/10.1021/acsami.6b13422.
Article CAS PubMed Google Scholar
Mohd Pu’ad NAS, Koshy P, Abdullah HZ, Idris MI, Lee TC. Syntheses of hydroxyapatite from natural sources. Heliyon. 2019;5(5):e01588. https://doi.org/10.1016/j.heliyon.2019.e01588.
Article PubMed PubMed Central Google Scholar
Nandi SK, Kundu B, Mukherjee J, Mahato A, Datta S, Balla VK. Converted marine coral hydroxyapatite implants with growth factors: in vivo bone regeneration. Mater Sci Eng C Mater Biol Appl. 2015;49:816–23. https://doi.org/10.1016/j.msec.2015.01.078.
Article CAS PubMed Google Scholar
Neto AS, Ferreira JMF. Synthetic and marine-derived porous scaffolds for bone tissue engineering. Materials (Basel). 2018;11(9):1702. https://doi.org/10.3390/ma11091702.
Article CAS PubMed Google Scholar
Pallela R, Venkatesan J, Janapala VR, Kim SK. Biophysicochemical evaluation of chitosan-hydroxyapatite-marine sponge collagen composite for bone tissue engineering. J Biomed Mater Res A. 2012;100(2):486–95. https://doi.org/10.1002/jbm.a.33292.
Article CAS PubMed Google Scholar
Palmer LC, Newcomb CJ, Kaltz SR, Spoerke ED, Stupp SI. Biomimetic systems for hydroxyapatite mineralization inspired by bone and enamel. Chem Rev. 2008;108(11):4754–83. https://doi.org/10.1021/cr8004422.
Article CAS PubMed PubMed Central Google Scholar
Panda NN, Pramanik K, Sukla LB. Extraction and characterization of biocompatible hydroxyapatite from fresh water fish scales for tissue engineering scaffold. Bioprocess Biosyst Eng. 2014;37(3):433–40. https://doi.org/10.1007/s00449-013-1009-0.
Article CAS PubMed Google Scholar
Piccirillo C, Pintado MM, Castro PM. Hydroxyapatite and calcium phosphates from marine sources: extraction and characterization. In: Kim SK, editor. Marine biomaterials: characterization, isolation and applications. Boca Raton: CRC Press; 2013.
Google Scholar
Piccirillo C, Pullar RC, Costa E, Santos-Silva A, Pintado MM, Castro PM. Hydroxyapatite-based materials of marine origin: a bioactivity and sintering study. Mater Sci Eng C Mater Biol Appl. 2015;51:309–15. https://doi.org/10.1016/j.msec.2015.03.020.
Article CAS PubMed Google Scholar
Radulescu DE, Neacsu IA, Grumezescu AM, Andronescu E. Novel trends into the development of natural hydroxyapatite-based polymeric composites for bone tissue engineering. Polymers (Basel). 2022;14(5):899. https://doi.org/10.3390/polym14050899.
Article CAS PubMed Google Scholar
Rodríguez-Valencia C, López-Álvarez M, Cochón-Cores B, Pereiro I, Serra J, González P Novel seleniumdoped hydroxyapatite coatings for biomedical applications. J Biomed Mater Res A 2013;101(3):853–861. https://doi.org/10.1002/jbm.a.34387
Romano G, Almeida M, Varela Coelho A, Cutignano A, Goncalves LG, Hansen E, Khnykin D, Mass T, Ramsak A, Rocha MS, Silva TH, Sugni M, Ballarin L, Geneviere AM. Biomaterials and bioactive natural products from marine invertebrates: from basic research to innovative applications. Mar Drugs. 2022;20(4):219. https://doi.org/10.3390/md20040219.
Article CAS PubMed PubMed Central Google Scholar
Schulze F, Lang A, Schoon J, Wassilew GI, Reichert J. Scaffold guided bone regeneration for the treatment of large segmental defects in long bones. Biomedicine. 2023;11(2):325. https://doi.org/10.3390/biomedicines11020325.
Article CAS Google Scholar
Scialla S, Carella F, Dapporto M, Sprio S, Piancastelli A, Palazzo B, Adamiano A, Degli Esposti L, Iafisco M, Piccirillo C. Mussel shell-derived macroporous 3D scaffold: characterization and optimization study of a bioceramic from the circular economy. Mar Drugs. 2020;18(6):309. https://doi.org/10.3390/md18060309.
Article CAS PubMed PubMed Central Google Scholar
Shavandi A, Bekhit AE-DA, Ali A, Sun Z. Synthesis of nano-hydroxyapatite (nHA) from waste mussel shells using a rapid microwave method. Mater Chem Phys. 2015;149–150:607–16. https://doi.org/10.1016/j.matchemphys.2014.11.016.
Article CAS Google Scholar
Sobczak-Kupiec A, Drabczyk A, Florkiewicz W, Glab M, Kudlacik-Kramarczyk S, Slota D, Tomala A, Tyliszczak B. Review of the applications of biomedical compositions containing hydroxyapatite and collagen modified by bioactive components. Materials (Basel). 2021;14(9):2096. https://doi.org/10.3390/ma14092096.
Article CAS PubMed Google Scholar
Surya P, Nithin A, Sundaramanickam A, Sathish M. Synthesis and characterization of nano-hydroxyapatite from Sardinella longiceps fish bone and its effects on human osteoblast bone cells. J Mech Behav Biomed Mater. 2021;119:104501. https://doi.org/10.1016/j.jmbbm.2021.104501.
Article CAS PubMed Google Scholar
Szychlinska MA, Bucchieri F, Fucarino A, Ronca A, D’Amora U. Three-dimensional bioprinting for cartilage tissue engineering: insights into naturally-derived bioinks from land and marine sources. J Funct Biomater. 2022;13(3):118.
Article CAS PubMed PubMed Central Google Scholar
Tae Young A, Kang JH, Kang DJ, Venkatesan J, Chang HK, Bhatnagar I, Chang KY, Hwang JH, Salameh Z, Kim SK, Kim HT, Kim DG. Interaction of stem cells with nano hydroxyapatite-fucoidan bionanocomposites for bone tissue regeneration. Int J Biol Macromol. 2016;93(Pt B):1488–91. https://doi.org/10.1016/j.ijbiomac.2016.07.027.
Article CAS PubMed Google Scholar
Teymouri A, Stuart BJ, Kumar S. Hydroxyapatite and dittmarite precipitation from algae hydrolysate. Algal Res. 2018;29:202–11. https://doi.org/10.1016/j.algal.2017.11.030.
Article Google Scholar
Venkatesan J, Anil S. Hydroxyapatite derived from marine resources and their potential biomedical applications. Biotechnol Bioprocess Eng. 2021;26(3):312–24. https://doi.org/10.1007/s12257-020-0359-0.
Article CAS Google Scholar
Venkatesan J, Kim SK. Effect of temperature on isolation and characterization of hydroxyapatite from tuna (Thunnus obesus) bone. Materials (Basel). 2010;3(10):4761–72. https://doi.org/10.3390/ma3104761.
Article CAS PubMed Google Scholar
Venkatesan J, Qian ZJ, Ryu B, Thomas NV, Kim SK. A comparative study of thermal calcination and an alkaline hydrolysis method in the isolation of hydroxyapatite from Thunnus obesus bone. Biomed Mater. 2011;6(3):035003. https://doi.org/10.1088/1748-6041/6/3/035003.
Article CAS PubMed Google Scholar
Venkatesan J, Rekha PD, Anil S, Bhatnagar I, Sudha PN, Dechsakulwatana C, Kim S-K, Shim MS. Hydroxyapatite from cuttlefish bone: isolation, characterizations, and applications. Biotechnol Bioprocess Eng. 2018;23(4):383–93. https://doi.org/10.1007/s12257-018-0169-9.
Article CAS Google Scholar
Xiao W, Fu H, Rahaman MN, Liu Y, Bal BS. Hollow hydroxyapatite microspheres: a novel bioactive and osteoconductive carrier for controlled release of bone morphogenetic protein-2 in bone regeneration. Acta Biomater. 2013;9(9):8374–83. https://doi.org/10.1016/j.actbio.2013.05.029.
Article CAS PubMed PubMed Central Google Scholar
Xu Y, Ye J, Zhou D, Su L. Research progress on applications of calcium derived from marine organisms. Sci Rep. 2020;10(1):18425. https://doi.org/10.1038/s41598-020-75575-8.
Article CAS PubMed PubMed Central Google Scholar
Yamamura H, da Silva VHP, Ruiz PLM, Ussui V, Lazar DRR, Renno ACM, Ribeiro DA. Physico-chemical characterization and biocompatibility of hydroxyapatite derived from fish waste. J Mech Behav Biomed Mater. 2018;80:137–42. https://doi.org/10.1016/j.jmbbm.2018.01.035.
Article CAS PubMed Google Scholar
Zhang K, Fan Y, Dunne N, Li X. Effect of microporosity on scaffolds for bone tissue engineering. Regen Biomater. 2018;5(2):115–24. https://doi.org/10.1093/rb/rby001.

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Department of Dentistry, Oral Health Institute, Hamad Medical Corporation, Doha, Qatar
Sukumaran Anil
College of Dental Medicine, Qatar University, Doha, Qatar
Sukumaran Anil
Pushpagiri Institute of Medical Sciences and Research Centre, Medicity, Tiruvalla, Kerala, India
Vishnupriya K. Sweety & Betsy Joseph
Saveetha Dental College and Hospital, Saveetha Institute of Medical and Technical Sciences, Chennai, India
Betsy Joseph

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Sukumaran Anil
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Vishnupriya K. Sweety
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Betsy Joseph
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3B’s Research Group, University of Minho, Barco, Portugal
Fatima Raquel Azevedo Maia
3B's Research Group, University of Minho, Guimarães, Portugal
J. Miguel Oliveira
Dept Polymer Engg, 3Bs Research Grp, University of Minho, Guimaraes, Portugal
Rui L. Reis

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Anil, S., Sweety, V.K., Joseph, B. (2023). Marine-Derived Hydroxyapatite for Tissue Engineering Strategies. In: Maia, F.R.A., Oliveira, J.M., Reis, R.L. (eds) Handbook of the Extracellular Matrix. Springer, Cham. https://doi.org/10.1007/978-3-030-92090-6_60-1

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DOI: https://doi.org/10.1007/978-3-030-92090-6_60-1
Received: 29 April 2023
Accepted: 02 May 2023
Published: 16 December 2023
Publisher Name: Springer, Cham
Print ISBN: 978-3-030-92090-6
Online ISBN: 978-3-030-92090-6
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