Abstract
Pullulan is the one of the most potent biocompatible polymer, which is basically synthesized by the Aureobasidium pullulans. This polymer appears to be a linear α-glucan of maltotriose units with occasional branching of glucosyl or maltosyl substitution. The employment and application of pullulan in biomedical and tissue engineering field is emerging owing to its biocompatible, nontoxic, non-immunogenic, and inert nature. It can be derivatized via various chemical reactions to increase its utility in the field of pharmaceuticals. In addition, pullulan and its derivatives have photographic, lithographic, and electronic applications in the biomedical instrumentation. This chapter provides comprehensive information about “pullulan” considering its microbial sources, biosynthesis aspects, characterization, and functionalization. It also highlights the various applications of pullulan and its derivatives in the pharmaceutical and biomedical fields.
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References
Bender H, Lehmann J, Wallenfels K. Pullulan, ein extracelluläres Glucan von Pullularia pullulans. Biochim Biophys Acta. 1959;36:309–16. https://doi.org/10.1016/0006-3002(59)90172-6.
Chen J, Wu S, Pan S. Optimization of medium for pullulan production using a novel strain of Auerobasidium pullulans isolated from sea mud through response surface methodology. Carbohydr Polym. 2012;87:771–4. https://doi.org/10.1016/j.carbpol.2011.08.062.
Chen L, Wang X, Ji F, Bao Y, Wang J, Wang X, Guo L, Li Y. New bifunctional-pullulan-based micelles with good biocompatibility for efficient co-delivery of cancer-suppressing p53 gene and doxorubicin to cancer cells. RSC Adv. 2015;5:94719–31.
Chen L, Qian M, Zhang L, Xia J, Bao Y, Wang J, Guo L, Li Y. Co-delivery of doxorubicin and shRNA of Beclin1 by folate receptor targeted pullulan-based multifunctional nanomicelles for combinational cancer therapy. RSC Adv. 2018;8:17710–22.
Cheng KC, Catchmark JM, Demirci A. Enhanced pullulan production in a biofilm reactor by using response surface methodology. J Ind Microbiol Biotechnol. 2010;37:587–94. https://doi.org/10.13031/2013.29694.
Chi Z, Wang F, Chi Z, Yue L, Liu G, Zhang T. Bioproducts from Aureobasidium pullulans, a biotechnologically important yeast. Appl Microbiol Biotechnol. 2009;82:793–804. https://doi.org/10.1007/s00253-009-1882-2.
Choudhury AR, Saluja P, Prasad G. Pullulan production by an osmotolerant Aureobasidium pullulans RBF-4A3 isolated from flowers of Caesulia axillaris. Carbohydr Polym. 2011;83:1547–52. https://doi.org/10.1016/j.carbpol.2010.10.003.
Constantin M, Bucătariu S, Stoica I, Fundueanu G. Smart nanoparticles based on pullulan-g-poly (N-isopropylacrylamide) for controlled delivery of indomethacin. Int J Biol Macromol. 2017;94:698–708. https://doi.org/10.1016/j.ijbiomac.2016.10.064.
Dailin DJ, Low LZMI, Kumar K, Malek RA, Natasya KH, Keat HC, Sukmawati D, Enshasy HE. Agro-industrial waste: a potential feedstock for pullulan production. Biosci Biotechnol Res Asia. 2019;16:229–50. https://doi.org/10.13005/bbra/2740.
Dubey KK. Insights of microbial pullulan production: a bioprocess engineer assessment. Current Biotechnol. 2018;7:262–72.
Eslaminejad T, Nematollahi-Mahani SN, Ansari M. Synthesis, characterization, and cytotoxicity of the plasmid EGFP-p53 loaded on pullulan–spermine magnetic nanoparticles. J Magn Magn Mater. 2016;402:34–43.
Fujioka-Kobayashi M, Ota MS, Shimoda A, K-i N, Akiyoshi K, Miyamoto Y, Iseki S. Cholesteryl group-and acryloyl group-bearing pullulan nanogel to deliver BMP2 and FGF18 for bone tissue engineering. Biomaterials. 2012;33:7613–20. https://doi.org/10.1016/j.biomaterials.2012.06.075.
Haifeng L, Yunyi Z, Yunyun G, Yuanyang L, Xiaopu Y, Lifeng H. Characterization of UGPase from Aureobasidium pullulans NRRLY-12974 and application in enhanced pullula production. Appl Biochem Biotechnol. 2016;178:1141–53. https://doi.org/10.1007/s12010-0151934-2.
Han Y, Lv S. Synthesis of chemically crosslinked pullulan/gelatin-based extracellular matrix-mimetic gels. Int J Biol Macromol. 2019;122:1262–70. https://doi.org/10.1016/j.ijbiomac.2018.09.080.
Hussain MA, Abbas K, Lodhi BA, Sher M, Ali M, Tahir MN, Tremel W, Iqbal S. Fabrication, characterization, thermal stability and nanoassemblies of novel pullulan-aspirin conjugates. Arab J Chem. 2017;10:1597–603.
Iswariya S, Bhanukeerthi A, Velswamy P, Uma T, Perumal PT. Design and development of a piscine collagen blended pullulan hydrogel for skin tissue engineering. RSC Adv. 2016;6:57863–71. https://doi.org/10.1039/c6ra03578g.
Jafari A, Sun H, Sun B, Mohamed MA, Cui H, Cheng C. Layer-by-layer preparation of polyelectrolyte multilayer nanocapsules via crystallized miniemulsions. Chem Commun. 2019;55:1267–70. https://doi.org/10.1039/c8cc08043g.
Kulkarni A, Madane T, Aloorkar N, Mujumdar S. Enhancement of solubility of BCS class II drug (pioglitazone) using natural polymer (pullulan). Current Pharma Res. 2019;9:3321–34.
Lepoittevin B, Elzein T, Dragoe D, Bejjani A, Lemee F, Levillain J, Bazin P, Roger P, Dez I. Hydrophobization of chitosan films by surface grafting with fluorinated polymer brushes. Carbohydr Polym. 2019;205:437–46. https://doi.org/10.1016/j.carbpol.2018.10.044.
Leung V, Mapletoft J, Zhang A, Lee A, Vahedi F, Chew M, Szewczyk A, Jahanshahi-Anbuhi S, Ang J, Cowbrough B. Thermal stabilization of viral vaccines in low-cost sugar films. Sci Rep. 2019;9:1–11. https://doi.org/10.1038/s41598-019-44020-w.
Liu J, J-i J, Kawai Y, Aoki I, Tanaka C, Yamamoto M, Tabata Y. Preparation of polymer-based multimodal imaging agent to visualize the process of bone regeneration. J Control Release. 2012;157:398–405. https://doi.org/10.1016/j.jconrel.2011.09.090.
Mishra B, Suneetha V. Biosynthesis and hyper production of pullulan by a newly isolated strain of Aspergillus japonicus-VIT-SB1. World J Microbiol Biotechnol. 2014;30:2045–52. https://doi.org/10.1007/s11274-014-1629-9.
Mishra B, Varjani S. Evaluation of pullulan production by a newly isolated Micrococcus luteus. Indian J Exp Biol. 2019;57:813–20.
Mishra B, Vuppu S, Rath K. The role of microbial pullulan, a biopolymer in pharmaceutical approaches: a review. J Appl Pharm Sci. 2011;01:45–50.
Mishra B, Manikanta A, Zamare D. Preparation of Maltotriose syrup from microbial pullulan by using pullulanase enzyme. Biosci Biotech Res Asia. 2016;13:481–5.
Mishra B, Zamare D, Manikanta A. Selection and utilization of agro-industrial waste for biosynthesis and hyper-production of pullulan: a review. In: Varjani S, Parameswaran B, Kumar S, Khare S, editors. Biosynthetic technology and environmental challenges. Energy, environment, and sustainability. Singapore: Springer; 2018. p. 89–103. https://doi.org/10.1007/978-981-10-7434-9_6.
Morimoto N, Winnik FM, Akiyoshi K. Botryoidal assembly of cholesteryl− pullulan/poly (N-isopropylacrylamide) nanogels. Langmuir. 2007;23:217–23. https://doi.org/10.1021/la0616045.
Shu CH, Lin KJ, Wen BJ. Effects of culture temperature on the production of bioactive polysaccharides by Agaricus blazei in batch cultures. J Chem Technol Biotechnol. 2007;82:831–6. https://doi.org/10.1002/jctb.1748.
Singh R, Saini G. Pullulan-hyperproducing color variant strain of Aureobasidium pullulans FB-1 newly isolated from phylloplane of Ficus sp. Bioresour Technol. 2008;99:3896–9. https://doi.org/10.1016/j.biortech.2007.08.003.
Singh RS, Saini GK, Kennedy JF. Downstream processing and characterization of pullulan from a novel colour variant strain of Aureobasidium pullulans FB-1. Carbohydr Polym. 2009;78:89–94. https://doi.org/10.1016/j.carbpol.2009.03.040.
Singh RS, Kaur N, Kennedy JF. Pullulan production from agro-industrial waste and its applications in food industry: a review. Carbohydr Polym. 2019;217:46–57. https://doi.org/10.1016/j.carbpol.2019.04.050.
Soto KM, Hernández-Iturriaga M, Loarca-Piña G, Luna-Bárcenas G, Mendoza S. Antimicrobial effect of nisin electrospun amaranth: pullulan nanofibers in apple juice and fresh cheese. Int J Food Microbiol. 2019;295:25–32. https://doi.org/10.1016/j.ijfoodmicro.2019.02.001.
Spatareanu A, Bercea M, Budtova T, Harabagiu V, Sacarescu L, Coseri S. Synthesis, characterization and solution behaviour of oxidized pullulan. Carbohydr Polym. 2014;111:63–71. https://doi.org/10.1016/j.carbpol.2014.04.060.
Sugumaran KR, Ponnusami V. Downstream processing studies for pullulan recovery in solid state fermentation using asian palmyra palm kernelinexpensive substrate. Biotechnology. 2014;9(2):79–82.
Sugumaran K, Ponnusami V. Review on production, downstream processing and characterization of microbial pullulan. Carbohydr Polym. 2017;173:573–91. https://doi.org/10.1016/j.carbpol.2017.06.022.
Sugumaran K, Sindhu R, Sukanya S, Aiswarya N, Ponnusami V. Statistical studies on high molecular weight pullulan production in solid state fermentation using jack fruit seed. Carbohydr Polym. 2013;98:854–60. https://doi.org/10.1016/j.carbpol.2013.06.071.
Takahata T, Okihara T, Yoshida Y, Yoshihara K, Shiozaki Y, Yoshida A, Yamane K, Watanabe N, Yoshimura M, Nakamura M. Bone engineering by phosphorylated-pullulan and β-TCP composite. Biomed Mater. 2015;10:065009.
Teekamp N, Tian Y, Visser JC, Olinga P, Frijlink HW, Woerdenbag HJ, Hinrichs WL. Addition of pullulan to trehalose glasses improves the stability of β-galactosidase at high moisture conditions. Carbohydr Polym. 2017;176:374–80. https://doi.org/10.1016/j.carbpol.2017.08.084.
Thirumavalavan K, Manikkadan T, Dhanasekar R. Pullulan production from coconut by-products by Aureobasidium pullulans. Afr J Biotechnol. 2009;8:254–8.
Tian Y, Visser JC, Klever JS, Woerdenbag HJ, Frijlink HW, Hinrichs WL. Orodispersible films based on blends of trehalose and pullulan for protein delivery. Eur J Pharm Biopharm. 2018;133:104–11.
Tiwari S, Bahadur P. Modified hyaluronic acid based materials for biomedical applications. Int J Biol Macromol. 2019;121:556–71. https://doi.org/10.1016/j.ijbiomac.2018.10.049.
Wang J, Cui S, Bao Y, Xing J, Hao W. Tocopheryl pullulan-based self assembling nanomicelles for anti-cancer drug delivery. Mater Sci Eng C. 2014;43:614–21. https://doi.org/10.1016/j.msec.2014.07.066.
Wang D, Bian J, Wei G, Jiang M, Dong M. Simultaneously enhanced production and molecular weight of pullulan using a two-stage agitation speed control strategy. J Chem Technol Biotechnol. 2016;91:467–75. https://doi.org/10.1002/jctb.4600.
Xiao Q, Tong Q, Lim L-T. Pullulan-sodium alginate based edible films 2012 rheological properties of film forming solutions. Carbohydr Polym. 2012;87:1689–95.
Yu X, Wang Y, Wei G, Dong Y. Media optimization for elevated molecular weight and mass production of pigment-free pullulan. Carbohydr Polym. 2012;89:928–34. https://doi.org/10.1016/j.carbpol.2012.04.038.
Zan Z, Zou X. Efficient production of polymalic acid from raw sweet potato hydrolysate with immobilized cells of Aureobasidium pullulans CCTCC M2012223 in aerobic fibrous bed bioreactor. J Chem Technol Biotechnol. 2013;88:1822–7. https://doi.org/10.1002/jctb.4033.
Zhou J, Mohamed Wali AR, Ma S, He Y, Yue D, Tang JZ, Gu Z. Tailoring the supramolecular structure of guanidinylated pullulan toward enhanced genetic photodynamic therapy. Biomacromolecules. 2018;19:2214–26.
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Reddy, C.N., Mishra, B., Mandal, S.K., Agrawal, D.C., Kruthiventi, C. (2022). An Insight into Pullulan and Its Potential Applications. In: Oliveira, J.M., Radhouani, H., Reis, R.L. (eds) Polysaccharides of Microbial Origin. Springer, Cham. https://doi.org/10.1007/978-3-030-42215-8_15
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DOI: https://doi.org/10.1007/978-3-030-42215-8_15
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