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Definition
Plasmid incompatibility refers to the inability of two plasmids that contain the same replicon to exist stably in an individual cell (del Solar et al. 1998; Novick 1987). A replicon is the specific DNA sequence required for replication of a particular plasmid. The replicon can include cis-acting sequences (e.g., protein-binding sites) and genes that encode diffusible molecules such as RNA or proteins that are necessary for plasmid replication. Two plasmids that contain the same replicon but that are otherwise different (i.e., they may contain different DNA inserts or different antibiotic resistance genes) will not be maintained together in a single bacterial cell in the absence of selective pressure for both plasmids. Instead, one or the other of the plasmids will be lost from the progeny of individual cells, so that the population of cells will after some time consist of cells that contain only one of the original plasmids.
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Plasmid...
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References
Chattoraj DK (2000) Control of plasmid DNA replication by iterons: no longer paradoxical. Mol Microbiol 37:467–476
del Solar G, Giraldo R, Ruiz-Echevarria MJ, Espinosa M, Diaz-Orejas R (1998) Replication and control of circular bacterial plasmids. Microbiol Mol Biol Rev 62:434–464
Lin-Chao S, Cohen SN (1991) The rate of processing and degradation of antisense RNAI regulates the replication of ColE1-type plasmids in vivo. Cell 65:1233–1242
Novick RP (1987) Plasmid incompatibility. Microbiol Rev 51:381–395
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Julin, D. (2015). Cloning Vector Compatibility. In: Bell, E. (eds) Molecular Life Sciences. Springer, New York, NY. https://doi.org/10.1007/978-1-4614-6436-5_92-4
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DOI: https://doi.org/10.1007/978-1-4614-6436-5_92-4
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Publisher Name: Springer, New York, NY
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Chapter history
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Latest
Cloning Vector Compatibility- Published:
- 18 February 2015
DOI: https://doi.org/10.1007/978-1-4614-6436-5_92-4
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Original
Plasmid Incompatibility- Published:
- 03 May 2014
DOI: https://doi.org/10.1007/978-1-4614-6436-5_92-3