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Antioxidant Activity and Capacity Measurement

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Plant Antioxidants and Health

Part of the book series: Reference Series in Phytochemistry ((RSP))

Abstract

Measurement of antioxidant activity/capacity of foods and biological fluids is important for the determination of nutritional value of foods and for the diagnosis of diseases associated with oxidative stress. Antioxidant activity/capacity can be monitored using assays with different mechanisms, including (i) electron transfer-based assays, (ii) hydrogen atom transfer-based assays, (iii) mixed-mode assays, (iv) lipid peroxidation assays, (v) ROS/RNS scavenging assays, (vi) oxidative stress biomarkers and cellular-based assays, and (vii) chromatographic, chemometric, and electrochemical assays. In this chapter, the mechanisms of the most widely used analytical approaches for the measurement of antioxidant activity/capacity are discussed together with their advantages and drawbacks. Overall, measurement of antioxidant activity/capacity, in particular for mixtures, multifunctional or complex multiphase systems, cannot be evaluated satisfactorily with a simple antioxidant assay due to the several variables affecting the results. Therefore, it is recommended to apply several assays with different mechanisms in order to reveal the full capability of an antioxidant sample.

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Authors and Affiliations

  1. Department of Food Engineering, Faculty of Chemical and Metallurgical Engineering, Istanbul Technical University, Maslak, Istanbul, Turkey

    Esra Capanoglu

  2. Science and Technology Application and Research Center (BITUAM), Bursa Uludag University, Gorukle, Bursa, Turkey

    Senem Kamiloglu

  3. Department of Chemistry, Faculty of Engineering, Istanbul University-Cerrahpasa, Avcilar, Istanbul, Turkey

    Sema Demirci Cekic, Kevser Sozgen Baskan, Asli Neslihan Avan, Seda Uzunboy & Resat Apak

  4. Turkish Academy of Sciences (TUBA), Cankaya, Ankara, Turkey

    Resat Apak

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  1. Department of Pharmaceutical Botany, Jagiellonian University, Medical College, Kraków, Poland

    Halina Maria Ekiert

  2. Department of Botany, Mohanlal Sukhadia University, Udaipur, India

    Kishan Gopal Ramawat

  3. Department of Botany, Mohanlal Sukhadia University, Udaipur, India

    Jaya Arora

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  1. Laboratory of Biomolecular Technology, Department of Botany, Mohanlal Sukhadia University, Udaipur, Rajasthan, India

    Jaya Arora

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Capanoglu, E. et al. (2021). Antioxidant Activity and Capacity Measurement. In: Ekiert, H.M., Ramawat, K.G., Arora, J. (eds) Plant Antioxidants and Health. Reference Series in Phytochemistry. Springer, Cham. https://doi.org/10.1007/978-3-030-45299-5_22-1

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