Abstract
This chapter describes the methodology and a detailed protocol for the targeting and optical imaging of experimental brain tumors in rats using quantum dots (QDs). QDs are optical semiconductor nanocrystals that exhibit stable, bright fluorescence over narrow, size-tunable emission bands. The size-tunable optical properties of QDs allow multiplexing with multiple emission wavelengths from a single excitation source. QDs may be linked to antibodies, peptides, and nucleic acids for use as fluorescence probes in vitro and in vivo. We have hypothesized that the intravenous injection of QDs may represent a novel technique to optically label brain tumors, potentially leading to improved techniques for surgical biopsy and resection.
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Lacroix, M., Abi-Said, D., Fourney, D. R., et al. (2001) A multivariate analysis of 416 patients with glioblastoma multiforme: prognosis, extent of resection, and survival. J. Neurosurg. 95, 190–198.
Toms, S. A., Ferson, D., and Sawaya, R. (1999) Basic surgical techniques in the resection of malignant gliomas. J. Neurooncol. 42, 215–226.
Stummer, W., Novotny, A., Stepp, H., Goetz, C., Bise, K., and Reulen, H. J. (2000) Fluorescence-guided resection of glioblastoma multiforme by using 5-aminole-vulinic acid-induced porphyrins: a prospective study in 52 consecutive patients. J. Neurosurg. 93, 1003–1013.
Kabuto, M., Kubata, T., Kobayashi, H., et al. (1997) Experimental and clinical study of detection of glioma at surgery using fluorescent imaging by a surgical microscope after fluoroscein administration. Neurol. Res. 19, 9–16.
Lin, W. C., Toms, S. A., Johnson, M., Jansen, E. D., and Mahadevan-Jansen, A. (2001) In vivo brain tumor demarcation using optical spectroscopy. Photochem. Photobiol. 73, 396–402.
Chan, W. C. and Nie, S. (1998) Quantum dot bioconjugates for ultrasensitive nonisotopic detection. Science 281, 2016–2018.
Bruchez, M., Jr., Morrone, M., Gin, P., Weis, S., and Alvisatos A., P. (1998) Semiconductor nanocrystals as fluorescent biological labels. Science 281, 2013–2016.
Ballou, B., Lagerholm, B. C., Ernst, L. A., Bruchez, M. P., and Waggoner, A. S. (2004) Noninvasive imaging of quantum dots in mice. Bioconjugate Chem. 15, 79–86.
West, J. L. and Halas, N. J. (2003) Engineered nanomaterials for biophotonics applications: improving sensing, imaging, and therapeutics. Annu. Rev. Biomed. Eng. 5, 285–292.
Michalet, X., Pinaud, F. F., Bentolilia, L. A., et al. (2005) Quantum dots for live cells, in vivo imaging, and diagnostics. Science 307, 538–544.
Gao, X., Yang, L., Petros, J. A., Marshall, F. F., Simons, J. W., and Nie, S. (2005) In vivo molecular and cellular imaging with quantum dots. Curr. Opin. Biotechnol. 16, 63–72.
Larson, D. R., Zipfel, W. R., Williams, R. M., et al. (2003) Water-soluble quantum dots for multiphoton fluorescence imaging in vivo. Science 300, 1434–1436.
Kim, S., Lin, Y. T., Soltesz, E. G., et al. (2004) Near-infrared fluorescent type II quantum dots for sentinel lymph node mapping. Nat. Biotechnol. 22, 93–97.
Corot, C., Petry, K. G., Trivedi, R., et al. (2004) Macrophage imaging in the central nervous system and in carotid atherosclerotic plaque using ultrasmall superparamagnetic iron oxide in magnetic resonance imaging. Invest. Radiol. 39, 619–625.
Vassallo, P., Matei, C., Heston, W. D., McLachlan, S. J., Koutcher, J. A., and Castellino, R. A. (1994) AMI-227-enhanced MR lymphangography: usefulness for differentiating reactive from tumor-bearing lymph nodes. Radiology 193, 501–506.
Dupas, B., Berreur, M., Rohanizadeh, R., Bonnemain, B., Meflah, K., and Prada, G. (1999) Electron microscopy study of intrahepatic ultrasmall superparamagnetic iron oxide kinetics in the rat. Relationship with magnetic resonance imaging. Bio. Cell 91, 195–208.
Kircher, M. F., Mahmood, U., King, R. S., Weissleder, R., and Josephson, L. (2003) A multimodal nanoparticle for preoperative magnetic resonance imaging and intraoperative optical brain tumor delineation. Ca. Res. 63, 8122–8125.
Zhang, Y., Kohler, N., and Zhang, M. (2002) Surface modifications of superparamagnetic magnetite nanoparticles and their intracellular uptake. Biomaterials 23, 1553–1561.
Moghimi, S. M. and Szebeni, J. (2003) Stealth liposomes and long circulating nanoparticles: critical issues in pharmacokinetics, opsonization, and protein-binding properties. Prog. Lipid Res. 42, 463–478.
Fleige, G., Nolte, C., Synowitz, M., Seeberger, F., Kettenmann, H., and Zimmer, C. (2001) Magnetic labeling of activated microglia in experimental glioma. Neoplasia 3, 489–499.
Hunt, M. A., Bago, A. G., and Neuwelt, E. A. (2005) Single dose contrast agent for intraoperative MR imaging of intrinsic brain tumors by using ferumoxtran-10. AJNR Am. J. Neuroradiol. 26, 1084–1088.
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© 2007 Humana Press Inc., Totowa, NJ
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Muhammad, O., Popescu, A., Toms, S.A. (2007). Macrophage-Mediated Colocalization of Quantum Dots in Experimental Glioma. In: Bruchez, M.P., Hotz, C.Z. (eds) Quantum Dots. Methods in Molecular Biology, vol 374. Humana Press. https://doi.org/10.1385/1-59745-369-2:161
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DOI: https://doi.org/10.1385/1-59745-369-2:161
Publisher Name: Humana Press
Print ISBN: 978-1-58829-562-0
Online ISBN: 978-1-59745-369-1
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