Quantum Dots

1. Front Matter

   Pages i-xii

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2. Imaging of Fixed Cells and Tissues

   1. Front Matter

      Pages 1-1

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   2. Immunofluorescent Labeling of Proteins in Cultured Cells With Quantum Dot Secondary Antibody Conjugates

      • Richard L. Ornberg, Hongjian Liu

      Pages 3-10

   3. Immunohistochemical Detection With Quantum Dots

      • Rizwan S. Akhtar, Cecelia B. Latham, Dario Siniscalco, Carlo Fuccio, Kevin A. Roth

      Pages 11-28

   4. Quantum Dots for Multicolor Tumor Pathology and Multispectral Imaging

      • Johbu Itoh, Robert Yoshiyuki Osamura

      Pages 29-42

   5. Light and Electron Microscopic Localization of Multiple Proteins Using Quantum Dots

      • Thomas J. Deerinck, Ben N. G. Giepmans, Benjamin L. Smarr, Maryann E. Martone, Mark H. Ellisman

      Pages 43-53

   6. Human Metaphase Chromosome FISH Using Quantum Dot Conjugates

      • Joan H. M. Knoll

      Pages 55-66

3. Imaging of Live Cells

   1. Front Matter

      Pages 67-67

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   2. Biotin-Ligand Complexes With Streptavidin Quantum Dots for In Vivo Cell Labeling of Membrane Receptors

      • Diane S. Lidke, Peter Nagy, Thomas M. Jovin, Donna J. Arndt-Jovin

      Pages 69-79

   3. Single Quantum Dot Tracking of Membrane Receptors

      • Cédric Bouzigues, Sabine Lévi, Antoine Triller, Maxime Dahan

      Pages 81-91

   4. Optical Monitoring of Single Cells Using Quantum Dots

      • Jyoti K. Jaiswal, Sanford M. Simon

      Pages 93-104

   5. Peptide-Mediated Intracellular Delivery of Quantum Dots

      • B. Christoffer Lagerholm

      Pages 105-112

   6. Multiple Cell Lines Using Quantum Dots

      • Paul G. Wylie

      Pages 113-123

   7. Measuring Cell Motility Using Quantum Dot Probes

      • Weiwei Gu, Teresa Pellegrino, Wolfgang J. Parak, Rosanne Boudreau, Mark A. Le Gros, A. Paul Alivisatos et al.

      Pages 125-131

4. Imaging of Live Animals

   1. Front Matter

      Pages 133-133

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   2. Quantum Dots for In Vivo Molecular and Cellular Imaging

      • Xiaohu Gao, Leland W. K. Chung, Shuming Nie

      Pages 135-145

   3. Sentinel Lymph Node Mapping With Type-II Quantum Dots

      • John V. Frangioni, Sang-Wook Kim, Shunsuke Ohnishi, Sungjee Kim, Moungi G. Bawendi

      Pages 147-159

   4. Macrophage-Mediated Colocalization of Quantum Dots in Experimental Glioma

      • Osman Muhammad, Alexandra Popescu, Steven A. Toms

      Pages 161-171

5. Flow Cytometry Applications

   1. Front Matter

      Pages 173-173

      PDF

   2. Application of Quantum Dots to Multicolor Flow Cytometry

      • Pratip K. Chattopadhyay, Joanne Yu, Mario Roederer

      Pages 175-184

Quantum Dots captures many diverse applications enabling utility in biological detection. Organized into five parts, the first two parts cover the use of QDs in imaging fixed and living cells (and tissues). Protocols are included for using QDs in routine (protein and structural cellular labeling), as well as enabling (single receptor trafficking, clinical pathology, correlative microscopy) applications. Part 3 shows early efforts aimed at using QDs in live animals. The final 2 parts demonstrate the versatility of QD technology in existing assay technology.

• FISH
• Fluoreszenz in situ-Hybridisierung
• Genotyp
• Microarray
• Vivo
• cell lines
• chromosome
• microscopy

From the reviews:

"...provides an excellent reference for protocols that can be utlized repeatedly to yield reliable performance from quantum dot-enabled applications." -Anticancer Research 27, May 2007

"Bruschez and Holtz have compiled an extremely useful set of applications and protocols in Quantum Dots, Applications in Biology. The protocols, written by the many leaders in QD imaging, cover a wide range of applications and provide detailed methodology for each application supported by excellent illustrations. This book certainly belongs on the shelf of anyone interested in biological imaging. … in summary, this illustrative compilation of well-written protocols on QD is a must have for both the expert and novice interested in biological imaging." (Neel Ghosh, ChemBioChem, Issue 9, 2008)

• Quantum Dot Corporation, Hayward

  Marcel P. Bruchez, Charles Z. Hotz

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