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Proteomic Analysis of Human Bladder Tissue Using SELDI® Approach Following Microdissection Techniques

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Laser Capture Microdissection

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 293))

Abstract

Lysing of a complete biopsy sample results in a mixture of desired and undesired proteins, reflecting the originating cell types. Therefore microdissecting tissue material is mandatory prior to sample lysis and all downstream applications of protein analysis (proteomics). The two most important dissecting methods for bladder tissue specimens are manual microdissection and laser microdissection. Sample transfer can further be separated into manual laser pressure catapulting (LPC) and laser capture microdissection (LCM). One of the possible downstream applications of protein analysis is surfaceenhanced laser desorption ionization (SELDI) time-of-flight mass spectrometry. The small quantities of tissue obtained by microdissection are sufficient for use in the SELDI technique.

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Author information

Authors and Affiliations

  1. Institute of Pathology, University Hospital Aachen, RWTH, Aachen, Germany

    Rene C. Krieg

  2. Institute of Pathology, University of Regensburg, Regensburg, Germany

    Nadine T. Gaisa

  3. Department of Anatomy, Physiology, and Genetics, Institute for Molecular Medicine, Uniformed Services University of the Health Sciences, Bethesda, MD

    Cloud P. Paweletz & Ruth Knuechel

Authors
  1. Rene C. Krieg
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  2. Nadine T. Gaisa
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  3. Cloud P. Paweletz
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  4. Ruth Knuechel
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Editor information

Editors and Affiliations

  1. Department of Pathology, University of Aberdeen, Aberdeen, UK

    Graeme I. Murray  & Stephanie Curran  & 

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© 2005 Humana Press Inc.

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Krieg, R.C., Gaisa, N.T., Paweletz, C.P., Knuechel, R. (2005). Proteomic Analysis of Human Bladder Tissue Using SELDI® Approach Following Microdissection Techniques. In: Murray, G.I., Curran, S. (eds) Laser Capture Microdissection. Methods in Molecular Biology™, vol 293. Humana Press. https://doi.org/10.1385/1-59259-853-6:255

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  • DOI: https://doi.org/10.1385/1-59259-853-6:255

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-260-5

  • Online ISBN: 978-1-59259-853-3

  • eBook Packages: Springer Protocols

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