Laser Capture Microdissection

Methods and Protocols

  • Graeme I. Murray
  • Stephanie Curran

Part of the Methods in Molecular Biology™ book series (MIMB, volume 293)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Introduction

    1. Front Matter
      Pages 1-1
    2. Stephanie Curran, Graeme I. Murray
      Pages 3-7
  3. Microdissection and DNA Analysis

  4. RNA and Gene Expression Studies Using Microdissected Cells

    1. Front Matter
      Pages 125-125
    2. Lise Mette, Stephen Hamilton-Dutoit
      Pages 127-138
    3. Patrick Micke, Arne Östman, Joakim Lundeberg, Fredrik Ponten
      Pages 151-166
    4. Ludger Fink, Rainer Maria Bohle
      Pages 167-185
    5. Jun-ya Kaimori, Masaru Takenaka, Kousaku Okubo
      Pages 209-220

About this book

Introduction

Use of laser microdissection has grown enormously to become a very powerful high-throughput technology essential to a range of downstream applications in pathology, cancer research, life sciences, medical diagnostics, and biotechnology. In Laser Capture Microdissection, expert laboratory researchers noted for their optimized methods describe in step-by-step detail not only how to successfully carry out tissue-based laser microdissection in their own laboratories using the different systems available, but also how to apply a wide range of sophisticated molecular technologies to the microdissected tissue samples. These readily reproducible methods make it possible to carry out contamination-free molecular analyses of specific types of normal and diseased cells. Highlights include gene expression profiling of primary tumor cell populations, RNA transcript amplification and GeneChip® microarrays, colorectal cancer proteomics, and proteomic analysis of human bladder tissue using the SELDI® approach. Applications of the microdissected tissue described encompass PCR, gene expression studies including microarray analysis, and proteomics. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and state-of-the-art, Laser Capture Microdissection provides a highly practical guide to successful tissue-based laser microdissection using available commercial systems and to the interpretation of the results using a wide range of sophisticated molecular technologies.

Keywords

DNA Microarray PCR Proteomics gene expression genes hybridization microorganisms

Editors and affiliations

  • Graeme I. Murray
    • 1
  • Stephanie Curran
    • 1
  1. 1.Department of PathologyUniversity of AberdeenAberdeenUK

Bibliographic information

  • DOI https://doi.org/10.1385/1592598536
  • Copyright Information Humana Press 2005
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-260-5
  • Online ISBN 978-1-59259-853-3
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book