Abstract
This method describes the use of subtilisin-catalyzed peptide condensation to form a 15-residue glycopeptide from two smaller synthetic peptides. A 12-residue peptide ester is synthesized by solid-phase peptide synthesis using a PAM-modified Rink amide resin that allows the formation of a peptide ester suitable for subtilisin ligation. The 12-residue acyl donor peptide ester is then ligated to a 3-residue acyl acceptor glycopeptide amide using subtilisin (EC 3.4.21.62) in a buffered mixture of water and DMF (1:9).
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Tolbert, T.J., Wong, CH. (2004). Subtilisin-Catalyzed Glycopeptide Condensation. In: Niemeyer, C.M. (eds) Bioconjugation Protocols. Methods in Molecular Biology™, vol 283. Humana Press, Totowa, NJ. https://doi.org/10.1385/1-59259-813-7:267
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DOI: https://doi.org/10.1385/1-59259-813-7:267
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-58829-098-4
Online ISBN: 978-1-59259-813-7
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