Bioconjugation Protocols

Strategies and Methods

  • Christof M. Niemeyer

Part of the Methods in Molecular Biology™ book series (MIMB, volume 283)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Antibody and Enzyme Conjugates

    1. Front Matter
      Pages 1-1
    2. Vladimir V. Shuvaev, Thomas Dziubla, Rainer Wiewrodt, Vladimir R. Muzykantov
      Pages 3-19
    3. Patrick S. Stayton, Zhongli Ding, Allan S. Hoffman
      Pages 37-43
    4. Margherita Morpurgo, Francesco M. Veronese
      Pages 45-70
    5. Robert F. Graziano, Paul Guptill
      Pages 71-85
  3. Nucleic Acid Conjugates

    1. Front Matter
      Pages 125-125
    2. Verena Beier, Andrea Bauer, Michael Baum, Jörg D. Hoheisel
      Pages 127-135
    3. Goran Pljevaljčić, Falk Schmidt, Alexander Peschlow, Elmar Weinhold
      Pages 145-161
    4. Florian Kukolka, Marina Lovrinovic, Ron Wacker, Christof M. Niemeyer
      Pages 181-196
    5. Kunihiro Kaihatsu, David R. Corey
      Pages 207-214
  4. Glycosyl and Lipid Conjugates

    1. Front Matter
      Pages 215-215
    2. Jürgen Kuhlmann
      Pages 217-220
    3. Ines Heinemann, Martin Völkert, Herbert Waldmann
      Pages 221-232

About this book

Introduction

Contemporary approaches to the synthesis of chemically modified proteins not only require efficient means to control conjugation and the specific site of attachment of the conjugated moiety, but also the effective use of recent developments in microarray technologies and nanobiotechnology. In Bioconjugation Protocols: Strategies and Methods, expert laboratorians update the classic methods and introduce valuable new approaches that go beyond basic conjugation techniques to include elements from advanced organic synthesis, molecular biology, and materials science. These readily reproducible methods cover the preparation of protein conjugates using covalent and noncovalent conjugation, the synthesis of nucleic acid conjugates using a variety of labeling techniques, and approaches to semisynthetic conjugates of proteins. Additional chapters address the biofunctionalization of inorganic surfaces, including the on-chip synthesis of peptide nucleic acids to generate microarrays for the high-throughput analysis of RNA and DNA, gold nanoparticles, and carbon nanotube probes for atomic force microscopy. All the protocols follow the successful Methods in Molecular Biology™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and highly practical, Bioconjugation Protocols: Strategies and Methods offers both novice and experienced researchers access to the broad array of techniques needed to master the semisynthesis of functional biomolecular reagents for a wide variety of applications-ranging from novel biomedical diagnostics, to powerful new therapeutics, to advanced biomaterials.

Editors and affiliations

  • Christof M. Niemeyer
    • 1
  1. 1.Fachbereich Chemie der Universität DortmundDortmundGermany

Bibliographic information

  • DOI https://doi.org/10.1385/1592598137
  • Copyright Information Humana Press 2004
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-098-4
  • Online ISBN 978-1-59259-813-7
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book