Abstract
Expressed protein ligation is a powerful tool for the generation of natively folded proteins composed of recombinantly generated and chemically synthesized polypeptides. Using this approach, we developed protocols for the production of prenylated and/or otherwise-labeled Rab GTPase. The protocols are generally applicable to most small GTPases that can be supplied with a variety of new chemical functionalities. We used semisynthetic fluorescently labeled Rab7 GTPase as a molecular probe to study protein-protein interactions with components of the prenylation machinery
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Durek, T., Goody, R.S., Alexandrov, K. (2004). In Vitro Semisynthesis and Applications of C-Terminally Modified Rab Proteins. In: Niemeyer, C.M. (eds) Bioconjugation Protocols. Methods in Molecular Biology™, vol 283. Humana Press, Totowa, NJ. https://doi.org/10.1385/1-59259-813-7:233
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DOI: https://doi.org/10.1385/1-59259-813-7:233
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-58829-098-4
Online ISBN: 978-1-59259-813-7
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