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Dioxygen-Dependent Metabolism of Nitric Oxide

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Nitric Oxide Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 279))

Abstract

Nitric oxide (NO) serves critical signaling, energetic, and toxic functions throughout the biosphere. NO steady-state levels and functions are controlled in part by NO metabolism or degradation. Dioxygen-dependent NO dioxygenases (EC 1.14.12.17) and dioxygen-independent NO reductases (EC 1.7.99.7) are being identified as major routes for NO metabolism in various life forms. Here we describe the use of the Clark-type NO electrode, mechanistic inhibitors, and nitrate/nitrite assays to measure, characterize, and identify major NO metabolic pathways and enzymes in bacteria, fungi, plants, mammalian cells, and organelles. The methods may prove to be particularly useful for mechanistic investigations and the development of inhibitors, inducers, and other novel NO-modulating therapeutics.

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Author information

Authors and Affiliations

  1. Division of Critical Care Medicine, Children's Hospital Medical Center, Cincinnati, OH

    Paul R. Gardner, Anne M. Gardner & Craig K. Hallstrom

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  1. Paul R. Gardner
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  2. Anne M. Gardner
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  3. Craig K. Hallstrom
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Editors and Affiliations

  1. Department of Physiology, College of Medicine, University of Tennessee, Memphis, TN

    Aviv Hassid

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© 2004 Humana Press Inc.

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Gardner, P.R., Gardner, A.M., Hallstrom, C.K. (2004). Dioxygen-Dependent Metabolism of Nitric Oxide. In: Hassid, A. (eds) Nitric Oxide Protocols. Methods in Molecular Biology™, vol 279. Humana Press. https://doi.org/10.1385/1-59259-807-2:133

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  • DOI: https://doi.org/10.1385/1-59259-807-2:133

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-237-7

  • Online ISBN: 978-1-59259-807-6

  • eBook Packages: Springer Protocols

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