Nitric Oxide Protocols

  • Aviv Hassid

Part of the Methods in Molecular Biology™ book series (MIMB, volume 279)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Reinhard Berkels, Svenja Purol-Schnabel, Renate Roesen
    Pages 1-8
  3. Yoshiichiro Kitamura, Hirosuke Kobayashi, Kazuo Tanishita, Kotaro Oka
    Pages 35-43
  4. Peter A. Steerenberg, Jan G. C. van Amsterdam
    Pages 45-68
  5. Xiaoxiang Zheng, Gangmin Ning, Dihui Hong, Mu Zhang
    Pages 69-80
  6. Hazel Sutherland, Olga Zolle, Roba Khundkar, Alec W. M. Simpson, Jonathan C. Jarvis, Stanley Salmons
    Pages 105-111
  7. Paul R. Gardner, Anne M. Gardner, Craig K. Hallstrom
    Pages 133-150
  8. Kulwant S. Aulak, Thomas Koeck, John W. Crabb, Dennis J. Stuehr
    Pages 151-165
  9. Andrew J. Gow, Christiana W. Davis, David Munson, Harry Ischiropoulos
    Pages 167-172
  10. Trinity J. Bivalacqua, Weiwen Deng, Hunter C. Champion, Wayne J. G. Hellstrom, Philip J. Kadowitz
    Pages 173-185
  11. Yoshikazu Maeda, Kazuyuki Shimada, Uichi Ikeda
    Pages 213-224
  12. Kathleen G. Raman, Richard A. Shapiro, Edith Tzeng, Melina R. Kibbe
    Pages 225-234
  13. Back Matter
    Pages 235-246

About this book

Introduction

Carrying on the high standards of the much-praised first edition of Nitric Oxide Protocols, Aviv Hassid has brought together a panel of expert researchers and clinician scientists to describe in step-by-step detail the latest methodologies for the measurement of nitric oxide--and the enzyme that produces it-in biological tissues and fluids. The authors take advantage of the latest methodologies for the quantitation of biological fluids and tissues, including capillary electrophoresis, microcoaxial electrodes, in vivo measurement of nitric oxide in exhaled air, confocal microscopy, gas chromatography, in situ hybridization, and real-time polymerase chain reaction. Chapters on the measurement of the novel products of nitric oxide, such as nitrated proteins, S-nitrosylated proteins, and dioxygen-dependent NO metabolism, are also included. Additional chapters address the expression of nitric oxide synthase via the use of viral vectors in gene therapy for erectile dysfunction and cancer, as well as in retrovirus, adenovirus, or adenoassociated virus-mediated expression of nitric oxide synthase in vivo. The protocols follow the successful Methods in Molecular Biology™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
State-of-the-art and highly practical, Nitric Oxide Protocols, 2nd ed., offers investigators and clinican/scientists a gold-standard collection of readily reproducible analytical techniques for measuring levels of nitric oxide and determining its manifold functions and effects.

Editors and affiliations

  • Aviv Hassid
    • 1
  1. 1.Department of Physiology, College of MedicineUniversity of TennesseeMemphis

Bibliographic information

  • DOI https://doi.org/10.1385/1592598072
  • Copyright Information Humana Press 2004
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-237-7
  • Online ISBN 978-1-59259-807-6
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book