Abstract
In this work we describe methods for the analysis of RNAs that have been edited by the double-strand RNA-specific adenosine deaminase, ADAR. These RNAs contain inosine residues that can be detected and quantified by a variety of approaches, including base hydrolysis and thin-layer chromatography, reverse transcription polymerase chain reaction, primer extension, and inosine-specific base cleavage. The most common method for the analysis of editing will be described here. This method involves complete hydrolysis of edited RNAs to nucleo-side monophosphates, followed by separation of the products using thin-layer chromatography.
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© 2004 Humana Press Inc., Totowa, NJ
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Zhang, Z., Carmichael, G.G. (2004). Methods for the Analysis of Adenosine-to-Inosine Editing in RNA. In: Schoenberg, D.R. (eds) mRNA Processing and Metabolism. Methods in Molecular Biology™, vol 257. Humana Press. https://doi.org/10.1385/1-59259-750-5:075
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DOI: https://doi.org/10.1385/1-59259-750-5:075
Publisher Name: Humana Press
Print ISBN: 978-1-58829-225-4
Online ISBN: 978-1-59259-750-5
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