mRNA Processing and Metabolism

Methods and Protocols

  • Daniel R. Schoenberg

Part of the Methods in Molecular Biology™ book series (MIMB, volume 257)

Table of contents

  1. Front Matter
    Pages i-xv
  2. Hemali P. Phatnani, Arno L. Greenleaf
    Pages 17-28
  3. Eric J. Wagner, Andrea Baines, Todd Albrecht, Robert M. Brazas, Mariano A. Garcia-Blanco
    Pages 29-46
  4. Klaus Hartmuth, Hans-Peter Vornlocher, Reinhard Lührmann
    Pages 47-64
  5. Zuo Zhang, Gordon G. Carmichael
    Pages 75-83
  6. Stephanie S. Tseng-Rogenski, Tien-Hsien Chang
    Pages 93-101
  7. Francisco J. Iborra, Dean A. Jackson, Peter R. Cook
    Pages 103-113
  8. Luiz O. F. Penalva, Scott A. Tenenbaum, Jack D. Keene
    Pages 125-134
  9. Julie Baron-Benhamou, Niels H. Gehring, Andreas E. Kulozik, Matthias W. Hentze
    Pages 135-153
  10. Yuri V. Svitkin, Nahum Sonenberg
    Pages 155-170
  11. Christian Thoma, Antje Ostareck-Lederer, Matthias W. Hentze
    Pages 171-180
  12. Naomi Bergman, Joseph Milone, Elizabeth J. Bates, Mateusz Opyrchal, Vivian Bellofatto, Jeffrey Wilusz
    Pages 181-192
  13. Devi Mukherjee, David T. Fritz, Walter J. Kilpatrick, Min Gao, Jeffrey Wilusz
    Pages 193-211
  14. Guiliang Tang, Phillip D. Zamore
    Pages 223-243
  15. Alicia M. Celotto, Brenton R. Graveley
    Pages 245-254

About this book

Introduction

mRNA processing is a key step in gene expression that effects all the proteins within the cell. In mRNA Processing and Metabolism: Methods and Protocols, world-renowned researchers bring together the latest techniques spanning the breadth of mRNA processing and metabolism. Drawing on recent advances in microscopy, whole genome sequencing, microarrays, mass spectrometry, fluorescent detection methodologies, and RNA interference, the authors offer readily reproducible methods for the cotranscriptional processing events that occur while the mRNA is engaged with elongating RNA polymerase II, with splicing and its biochemical analysis and with alternative splicing. Additional methods cover mRNA export, the recovery and analysis of mRNP complexes, cytoplasmic translation, mRNA degradation in vivo and in vitro, and the controversial concept of nuclear translation. A variety of model organisms are used, including yeast, Drosophila, Xenopus, mice, plants, and cultured mammalian cells. Each proven protocol is described in step-by-step detail and contains an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Timely and authoritative, mRNA Processing and Metabolism: Methods and Protocols provides a powerful collection of novel techniques that are not only effective, but also immediately applicable to current problems in many areas of biological research.

Editors and affiliations

  • Daniel R. Schoenberg
    • 1
  1. 1.Department of Molecular and Cellular BiochemistryThe Ohio State UniversityColumbus

Bibliographic information

  • DOI https://doi.org/10.1385/1592597505
  • Copyright Information Humana Press 2004
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-225-4
  • Online ISBN 978-1-59259-750-5
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book