Abstract
Dye-ligand affinity is based on the ability of the reactive dyes to bind proteins in a selective and reversible manner (1,2). The dyes are generally either monochlorotriazine compounds (two example structures are shown in Fig. 1) and were originally developed in the textile industry. The reactive chloro group allows easy immobilization of the triazine dye to a support matrix, such as Sepharose or agarose, and, more recently, to nylon membranes.
(A) Structure of cibracon blue F3G-A (procion blue H-B, Reactive Blue 2) and (B) structure of procion red HE-3B (reactive red 120).
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© 2004 Humana Press Inc., Totowa, NJ
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McGettrick, A.F., Worrall, D.M. (2004). Dye-Ligand Affinity Chromatography. In: Cutler, P. (eds) Protein Purification Protocols. Methods in Molecular Biology, vol 244. Humana Press. https://doi.org/10.1385/1-59259-655-X:151
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DOI: https://doi.org/10.1385/1-59259-655-X:151
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