Book Volume 244 2004

Protein Purification Protocols

Editors:

ISBN: 978-1-58829-067-0 (Print) 978-1-59259-655-3 (Online)

Table of contents (44 protocols)

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  1. Front Matter

    Pages i-xii

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    Protocol

    Pages 1-13

    General Strategies

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    Protocol

    Pages 15-20

    Preparation of Extracts From Animal Tissues

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    Protocol

    Pages 21-27

    Protein Extraction From Plant Tissues

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    Protocol

    Pages 29-35

    Extraction of Recombinant Protein From Bacteria

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    Protocol

    Pages 37-46

    Protein Extraction From Fungi

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    Protocol

    Pages 47-52

    Subcellular Fractionation of Animal Tissues

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    Protocol

    Pages 53-63

    Subcellular Fractionation of Plant Tissues

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    Protocol

    Pages 65-74

    The Extraction of Enzymes From Plant Tissues Rich in Phenolic Compounds

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    Protocol

    Pages 75-84

    Avoidance of Proteolysis in Extracts

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    Protocol

    Pages 85-90

    Concentration of Extracts

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    Protocol

    Pages 91-99

    Making and Changing Buffers

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    Protocol

    Pages 101-116

    Purification and Concentration by Ultrafiltration

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    Protocol

    Pages 117-124

    Bulk Purification by Fractional precipitation

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    Protocol

    Pages 125-131

    Ion-Exchange Chromatography

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    Protocol

    Pages 133-138

    Hydrophobic Interaction Chromatography

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    Protocol

    Pages 139-149

    Affinity Chromatography

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    Protocol

    Pages 151-157

    Dye-Ligand Affinity Chromatography

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    Protocol

    Pages 159-166

    Lectin Affinity Chromatography

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    Protocol

    Pages 167-177

    Immunoaffinity Chromatography

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    Protocol

    Pages 179-190

    Immobilized Metal-Ion Affinity Chromatography

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