Abstract
Introduction of DNA into plant protoplasts via electroporation is a well-known procedure. By giving electrical impulses of high field strength, the cell membrane is reversibly permeabilized, so that DNA molecules can be introduced into the cell (1).
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References
Fromm, M. E., Morrish, F, Armstrong, C. Williams, R, Thomas, J, and Klein, T M. (1990) Inheritance and expression of chimeric genes in the progeny of transgenic maize plants. Bio/Technology 8, 833–839
Rhodes, C. A., Pierce, D A, Mettler, I. J., Mascarenhas, D., and Detmer, J. J (1988) Genetically transformed maize plants from protoplasts Science 240, 204–207.
Dekeyser, R A, Claes, B., De Rycke, R M U, Habets, M. E., Van Montagu, M C, and Caplan, A. B. (1990) Transient gene expression in intact and organized rice tissues. Plant Cell 2, 591–602.
D’Halluin, K., Bonne, E., Bossut, M, De Beuckeleer, M. and Leemans, J (1992) Transgenic maize plants by tissue electroporation Plant Cell 4, 1495–1505
Xiayi, K., Xiuwen, Z., Heping, S., and Baojian, L. (1996) Electroporation of immature maize zygotic embryos and regeneration of transgenic plants. Transgenic Res. 5, 219–221.
Laursen, C M, Krzyzek, R. A, Flick, C E, Anderson, P C, and Spencer, T M. (1994) Production of fertile transgenic maize by electroporation of suspension culture cells Plant Mol Biol 24, 51–61
Pescitelli, S. M. and Sukhapinda, K (1995) Stable transformation via electroporation into maize Type II callus and regeneration of fertile transgenic plants. Plant Cell Rep. 14, 712–716
Songstad, D. D., Halaka, F. G., DeBoer, D. L, Armstrong, C. L., Hinchee, M. A. W., Ford-Santino, C. G., et al (1993) Transient expression of GUS and anthocyanin constructs in intact maize immature embryos following electroporation. Plant Cell, Tissue Organ Cult 33, 195–201.
Sabn, N., Pelissier, B, and Teissie, J. (1996) Transient and stable electrotrans-formations of intact black Mexican sweet maize cells are obtained after preplasmolysis Plant Cell Rep 15, 924–928.
Chu, C. C, Wang, C C, Sun, C. S., Hsu, C, Yin, K C, Chu, C. Y, et al (1975) Establishment of an efficient medium for anther culture of rice through comparative experiments on the nitrogen sources Sci Sin 18, 659–668
Frearson, E. M., Power, J B., and Cocking, E. C. (1973) The isolation, culture and regeneration of Petunia leaf protoplasts Dev Biol 33, 130–137.
Murashige, T. and Skoog, F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15, 473–497
De Block, M., Botterman, J, Vandewiele, M., Dockx, J, Thoen, C, Gosselé, V., et al. (1987) Engineering herbicide resistance in plants by expression of a detoxifying enzyme. EMBO J 6, 2513–2518
Matzke, M A and Matzke, A J M (1995) How and why do plants inactivate homologous (trans)genes? Plant Physiol 107, 679–685
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© 1999 Humana Press Inc., Totowa, NJ
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D’Halluin, K., Bonne, E., Bossut, M., Le Page, R. (1999). Transformation of Maize via Tissue Electroporation. In: Hall, R.D. (eds) Plant Cell Culture Protocols. Methods In Molecular Biology™, vol 111. Humana Press. https://doi.org/10.1385/1-59259-583-9:367
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DOI: https://doi.org/10.1385/1-59259-583-9:367
Publisher Name: Humana Press
Print ISBN: 978-0-89603-549-2
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