Analysis of F2-Isoprostanes by Gas Chromatography-Mass Spectrometry/Negative Ion Chemical Ionization

  • L. Jackson RobertsII
  • Jason D. Morrow
Part of the Methods in Pharmacology and Toxicology book series (MIPT)

Abstract

Numerous methods have been developed to assess oxidative stress/injury in vivo in humans, which include products of oxidation of lipids, proteins, and DNA. However, it has long been recognized that most methods are unreliable because they measure products that are not specific products of free radical-induced oxidation, because the method is not specific for the oxidation product being measured, or because the method is too invasive for use in humans (1). Thus, it has long been recognized that there has been a need for a reliable method to assess oxidative stress status in vivo in humans.

Keywords

Oxidative Stress Status Phosphomolybdic Acid Glass Centrifuge Tube Glass Scintillation Vial Pentafluorobenzyl Bromide 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Humana Press Inc.,Totowa, NJ 2003

Authors and Affiliations

  • L. Jackson RobertsII
    • 1
  • Jason D. Morrow
    • 2
  1. 1.Departments of Pharmacology and MedicineVanderbilt UniversityNashville
  2. 2.Departments of Medicine and PharmacologyVanderbilt UniversityNashville

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