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Methods in Biological Oxidative Stress

  • Kenneth Hensley
  • Robert A. Floyd

Part of the Methods in Pharmacology and Toxicology book series (MIPT)

Table of contents

  1. Front Matter
    Pages i-xv
  2. Lipids

  3. DNA, Protein, and Amino Acids

    1. Front Matter
      Pages 77-77
    2. Jun Nakamura, James A. Swenberg
      Pages 109-115
    3. Nathalie Bernoud-Hubac, Sean S. Davies, Olivier Boutaud, L. Jackson Roberts II
      Pages 117-125
    4. Sean S. Davies, Cynthia J. Brame, Olivier Boutaud, Nathalie Bernoud-Hubac, L. Jackson Roberts II
      Pages 127-136
    5. Kelly S. Williamson, Kenneth Hensley, Quentin N. Pye, Scott Ferrell, Robert A. Floyd
      Pages 137-149
    6. Kelly S. Williamson, Kenneth Hensley, Robert A. Floyd
      Pages 151-157
  4. Reactive Oxygen Species and Reactive Nitrogen Species

    1. Front Matter
      Pages 159-159
    2. Alessandra Castegna, Jennifer Drake, Chava Pocernich, D. Allan Butterfield
      Pages 161-168
    3. Kenneth Hensley, Kelly S. Williamson, Robert A. Floyd
      Pages 169-174
    4. Alexander Christov, Ladan Hamdheydari, Paula Grammas
      Pages 175-184
    5. Kenneth Hensley, Shenyun Mou, Quentin N. Pye
      Pages 185-193
    6. Kenneth Hensley, Kelly S. Williamson
      Pages 195-199
    7. Kenneth Hensley, Yashige Kotake, Danny R. Moore, Hong Sang, Lester A. Reinke
      Pages 201-206
  5. Back Matter
    Pages 207-215

About this book

Introduction

Free radicals and reactive oxidizing agents are now recognized as causes or contributing factors in inflammation and other disease states and have become a major focus of interest in pharmacology, toxicology, physiology, and biochemistry. In Methods in Biological Oxidative Stress, expert researchers detail their best experimental methods for detecting free radicals and reactive oxygen species or their byproducts. The techniques range from established standard protocols to advanced methodologies using HPLC, mass spectrometry, and electron paramagnetic resonance. The HPLC techniques are applied to the electrochemical measurement of protein oxidation products, particularly nitrotyrosine and dityrosine, and to the electrochemical detection of DNA oxidation products. There are also mass spectrometry methods for measuring lipid oxidation products. Each technique is described in step-by-step detail to ensure robust results, with many presented by the experimenters who have led their development. Useful notes explain the pitfalls to avoid and the chemical principles underlying each method.
Authoritative and highly practical, Methods in Biological Oxidative Stress provides laboratory researchers and technicians a unique collection of standard, easy-to-use experimental methods for oxidative stress bioanalysis, plus cutting-edge instrumental techniques using HPLC, mass spectrometry, and EPR spectrometry.

Editors and affiliations

  • Kenneth Hensley
    • 1
  • Robert A. Floyd
    • 1
  1. 1.Free Radical Biology and Aging Research ProgramOklahoma Medical Research FoundationOklahoma City

Bibliographic information

  • DOI https://doi.org/10.1385/1592594247
  • Copyright Information Humana Press 2003
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-815-8
  • Online ISBN 978-1-59259-424-5
  • Series Print ISSN 1557-2153
  • Series Online ISSN 1940-6053
  • Buy this book on publisher's site