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Electrophysiological Studies of Ion Channels and Therapeutic Potential With Adenovirus-Mediated Gene Transfer

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Membrane Transporters

Part of the book series: Methods in Molecular Biology ((MIMB,volume 227))

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Abstract

Transmembrane ion channels, or, more broadly, membrane transporters, are the subject of electrophysiology that leads us to the understanding of nearly every event of biological activities. Since the introduction of patch clamp concepts and techniques (1,2), transmembrane ion channels have been extensively studied at tissue and cell levels in terms of biophysical, electrophysiological, and pharmacological properties.

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References

  1. Hodgkin, A. L. and Huxley, A. F. (1952) A quantitative description of membrane current and its application to conduction and excitation in nerve. J. Physiol. (Lond.) 117, 500–544.

    CAS  Google Scholar 

  2. Hamill, O. P., Marty, A., Neher, E., et al. (1981) Improved patch-clamp techniques for high-resolution current recording from cell and cell-free membrane patches. Pflugers Arch. 391, 85–100.

    Article  PubMed  CAS  Google Scholar 

  3. Drews, J. (2000) Drug discovery: a historical perspective. Science 287, 1960–1964.

    Article  PubMed  CAS  Google Scholar 

  4. Mountain, A. (2000) Gene therapy: the first decade. Trends Biotechnol. 18, 119–128.

    Article  PubMed  CAS  Google Scholar 

  5. Khuri, F. R., Nemunaitis, J., Ganly, I., et al. (2000) A controlled trial of intratumoral ONYX-015: a selectively-replicating adenovirus, in combination with cisplatin and 5-fluorouracil in patients with recurrent head and neck cancer. Nature Med. 6, 879–885.

    Article  PubMed  CAS  Google Scholar 

  6. High, K. A. (2001) Gene transfer as an approach to treating hemophilia. Circ. Res. 88, 137–144.

    PubMed  CAS  Google Scholar 

  7. Isner, J.M. (2002) Myocardial gene therapy. Nature 415, 234–239.

    Article  PubMed  CAS  Google Scholar 

  8. Johns, D. C., Marx, R., Mains, R. E., et al. (1999) Inducible genetic suppression of neuronal excitability. J. Neurosci. 19, 1691–1697.

    PubMed  CAS  Google Scholar 

  9. Qu, J., Barbuti, A., Protas, L., et al. (2001) HCN2 overexpression in newborn and adult ventricular myocytes: distinct effects on gating and excitability. Circ. Res. 89, e8–e14.

    Article  PubMed  CAS  Google Scholar 

  10. Ludwig, A., Zong, X., Jeglitsch, M., et al. (1998) A family of hyperpolarization-activated mammalian cation channels. Nature 393, 587–591.

    Article  PubMed  CAS  Google Scholar 

  11. Santoro, B., Liu, D. T., Yao, H., et al. (1998) Identification of a gene encoding a hyperpolarization-activated pacemaker channel of brain. Cell 93, 1–20.

    Article  Google Scholar 

  12. Graham, F. L. (2000) Adenovirus vectors for high-efficiency gene transfer into mammalian cells. Immunol. Today 21, 426–428.

    Article  PubMed  CAS  Google Scholar 

  13. Ng, P., Parks, R. J., Cummings, D. T., et al. (1999) A high efficiency Cre/loxP based system for construction of adenoviral vectors. Hum. Gene Ther. 10, 2667–2672.

    Article  PubMed  CAS  Google Scholar 

  14. Ng, P., Parks, R. J., Cummings, D. T., et al. (2000) An enhanced system for construction of adenovirus vectors by the two-plasmid rescue method. Hum. Gene Ther. 11, 693–699.

    Article  PubMed  CAS  Google Scholar 

  15. Ghavami, A., Baruscotti, M., Robinson, R. B., et al. (1997) Adenovirus-mediated expression of 5-HT1B receptors in cardiac ventricle myocytes: coupling to inward rectifying K+ channels. Eur. J. Pharmacol. 340, 259–266.

    Article  PubMed  CAS  Google Scholar 

  16. Kuznetsov, V., Pak, E., Robinson, R. B., et al. (1995) B2-Adrenergic receptor actions in neonatal and adult rat ventricular myocytes. Circ. Res. 76, 40–52.

    PubMed  CAS  Google Scholar 

  17. Lue, W. M. and Boyden, P. A. (1992) Abnormal electrical properties of myocytes from chronically infarcted canine heart: alteration in V max and the transient outward current. Circulation 85, 1175–1188.

    PubMed  CAS  Google Scholar 

  18. Neher, E. and Sakmann, B. (1992) The patch clamp technique. Sci. Am. 266, 44–51.

    Article  PubMed  CAS  Google Scholar 

  19. Qu, J., Cohen, I. S., and Robinson, R. B. (2000) Sympathetic innervation alters activation of pacemaker current (I f) in rat ventricles. J. Physiol. (Lond.) 526, 561–569.

    Article  CAS  Google Scholar 

  20. Santoro, B., Chen, S., Luthi, A., et al. (2000) Molecular and functional heterogeneity of hyperpolarization-activated pacemaker channels in the mouse CNS. J. Neurosci. 20, 5264–5275.

    PubMed  CAS  Google Scholar 

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© 2003 Humana Press Inc., Totowa, NJ

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Qu, J., Pham, T.V., Obreztchikova, M.N. (2003). Electrophysiological Studies of Ion Channels and Therapeutic Potential With Adenovirus-Mediated Gene Transfer. In: Yan, Q. (eds) Membrane Transporters. Methods in Molecular Biology, vol 227. Humana Press. https://doi.org/10.1385/1-59259-387-9:179

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  • DOI: https://doi.org/10.1385/1-59259-387-9:179

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-104-2

  • Online ISBN: 978-1-59259-387-3

  • eBook Packages: Springer Protocols

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