Membrane Transporters

Methods and Protocols

  • Qing Yan

Part of the Methods in Molecular Biology book series (MIMB, volume 227)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Wolfgang Busch, Milton H. Saier Jr.
    Pages 21-36
  3. Renae M. Ryan, Ann D. Mitrovic, Robert J. Vandenberg
    Pages 97-107
  4. Frances J. Sharom, Paula L. Russell, Qin Qu, Peihua Lu
    Pages 109-128
  5. Frances J. Sharom, Paul D. W. Eckford
    Pages 129-153
  6. Ana M. Ojeda, Dawn T. Bravo, Terry L. Hart, Stanley M. Parsons
    Pages 155-177
  7. David V. Pow, Robert Sullivan, Heather Scott
    Pages 213-244
  8. Stefan Bröer
    Pages 245-257
  9. Frederick B. Loiselle, Joseph R. Casey
    Pages 259-280
  10. Stanley J. Opella
    Pages 307-320
  11. Thomas A. Wilkinson, Yuan Chen
    Pages 321-334
  12. Stella W. Y. Tsang, Peter M. Haney
    Pages 351-358

About this book

Introduction

Membrane transporters and channels play key roles in drug absorption and distribution, influence drug efficacy and toxicity, and are the target of about 30% of currently marketed drugs. In Membrane Transporters: Methods and Protocols, a panel of recognized basic and clinical investigators-many world-famous-describe their proven, cutting-edge methodologies for studying these important proteins. Treated in step-by-step detail, these techniques take advantage of all the latest developments in biomedical research, including pharmacogenomics, bioinformatics, and microarray technologies. The authors explain databases and tools for bioinformatics analysis, provide practical guidelines for microarray experiments and data analysis, and illustrate the use of small-angle X-ray scattering, nuclear magnetic resonance (NMR), and molecular modeling to study the structural biology of membrane transporters. Methods for exploring structure-function correlation, such as site-directed mutagenesis, immunocytochemistry, and confocal microscopy, are also described, along with several methods that may help in the development of novel therapeutics. Each technique contains hands-on instructions, many notes on avoiding the problems that one may encounter, and suggestions for variant procedures.
Comprehensive and highly practical, Membrane Transporters: Methods and Protocols offers investigators working in biopharmaceutical science, drug discovery, and drug delivery both an array of powerful tools for work on membrane transporters and an essential guide to the state-of-the-art in the membrane transporter field today.

Editors and affiliations

  • Qing Yan
    • 1
  1. 1.MedImmune Inc.Mountain View

Bibliographic information

  • DOI https://doi.org/10.1385/1592593879
  • Copyright Information Humana Press 2003
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-104-2
  • Online ISBN 978-1-59259-387-3
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book