Abstract
The intracellular expression of recombinant single-chain Fv (scFv) molecules—termed intrabodies—has potential advantages for therapeutic use (1,2). These single polypeptide chains are made of one heavy-chain variable region (VH) linked through a flexible spacer, usually a repeated motif of 3× GGGGS (although some others have also been described) (3), to one light chain variable region (VL). The VH-linker-VL sequence can be inverted with no loss of the binding. Shortened linkers have been used to produce scFvs of divalent or bispecific forms (3,4).
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Nam, C.H., Moutel, S., Teillaud, JL. (2002). Generation of Murine scFv Intrabodies from B-Cell Hybridomas. In: O’Connell, J. (eds) RT-PCR Protocols. Methods in Molecular Biology, vol 193. Humana Press. https://doi.org/10.1385/1-59259-283-X:301
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DOI: https://doi.org/10.1385/1-59259-283-X:301
Publisher Name: Humana Press
Print ISBN: 978-0-89603-875-2
Online ISBN: 978-1-59259-283-8
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