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RT-PCR Protocols

  • Joe O’Connell

Part of the Methods in Molecular Biology book series (MIMB, volume 193)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Introduction

    1. Front Matter
      Pages 1-1
    2. Joe O’Connell
      Pages 3-17
    3. Joe O’Connell
      Pages 19-25
  3. Highly Sensitive Detection and Analysis of mRNA

    1. Front Matter
      Pages 27-27
    2. Susanne Greber-Platzer, Brigitte Balcz, Christine Fleischmann, Gert Lubec
      Pages 29-58
    3. Triona Goode, Wen-Zhe Ho, Terry O’Connor, Sandra Busteed, Steven D. Douglas, Fergus Shanahan et al.
      Pages 65-79
  4. Quantitative RT-PCR

    1. Front Matter
      Pages 81-81
    2. Caroline Joyce
      Pages 83-92
    3. Joe O’Connell, Aileen Houston, Raymond Kelly, Darren O’Brien, Aideen Ryan, Michael W. Bennett et al.
      Pages 93-102
    4. Franz Bachmair, Christian G. Huber, Guenter Daxenbichler
      Pages 103-116
    5. Kaisa Nieminen, Markus Halminen, Matti Waris, Mika Mäkelä, Johannes Savolainen, Minna Sjöroos et al.
      Pages 117-127
  5. Detection and Analysis of RNA Viruses

    1. Front Matter
      Pages 149-149
    2. Brian Mullan, Liam J. Fanning, Fergus Shanahan, Daniel G. Sullivan
      Pages 171-188
  6. In Situ Localization of mRNA Expression

    1. Front Matter
      Pages 189-189
    2. Yi Cao
      Pages 191-196
    3. Tatjana Crnogorac-Jurcevic, Torsten O. Nielsen, Nick R. Lemoine
      Pages 197-204
    4. Paul Ryan, Simon Aarons, Michael W. Bennett, Gary Lee, Gerald C. O’Sullivan, Joe O’Connell et al.
      Pages 205-211
  7. Differential mRNA Expression

    1. Front Matter
      Pages 225-225
    2. Valentina I. Shustova, Stephen J. Meltzer
      Pages 227-236
  8. Genetic Analysis

    1. Front Matter
      Pages 251-251
    2. Frank Waldron-Lynch, Claire Adams, Michael G. Molloy, Fergal O’Gara
      Pages 253-263
  9. RT-PCR in Immunology

    1. Front Matter
      Pages 265-265
    2. Nadège Gruel, Beatrix Kotlan, Marie Beuzard, Jean-Luc Teillaud
      Pages 281-300
    3. Chang Hoon Nam, Sandrine Moutel, Jean-Luc Teillaud
      Pages 301-327
    4. Jean Marchand, Pierre Carayon
      Pages 329-337
  10. RT-PCR in Anti-Sense Technology

    1. Front Matter
      Pages 339-339
    2. Michael C. Yeung, Allan S. Lau
      Pages 341-346
  11. RT-PCR in cDNA Cloning

  12. Back Matter
    Pages 375-378

About this book

Introduction

RT-PCR, the most widely used technique in biomedical research for analyzing gene expression, has proven a significant boon to the molecular investigation of disease pathogenesis. In RT-PCR Protocols, Joe O'Connell has assembled a panel of highly regarded molecular biologists and clinical researchers to describe in detail their most novel, useful, and interesting RT-PCR applications. Here the newcomer will find readily reproducible protocols for highly sensitive detection and quantification of gene expression, the in situ localization of gene expression in tissue, and the cloning of genes, as well as for analyzing T-cell clones and the differential expression of genes. For the expert seeking to extend the usefulness of RT-PCR, there are user-friendly applications that complement the latest technological advances, including laser-capture microdissection (LCM), real-time and quantitative PCR, microarray technology, cDNA cloning, and antibody engineering. Each protocol emphasizes the technical steps critical for experimental success and includes tips on avoiding potential pitfalls, notes explaining the method's scientific basis and its possible sources of error, and comments on how it might best be accommodated to various experimental circumstances.
Diverse and wide-ranging, RT-PCR Protocols offers investigators powerful techniques to be used in the molecular investigation of disease pathogenesis and shows how RT-PCR can complement other technological advances in the design of today's new therapeutic strategies.

Editors and affiliations

  • Joe O’Connell
    • 1
  1. 1.Department of MedicineNational University of IrelandCorkIreland

Bibliographic information

  • DOI https://doi.org/10.1385/159259283X
  • Copyright Information Humana Press 2002
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-875-2
  • Online ISBN 978-1-59259-283-8
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site