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Perforated Patch-Clamp Technique

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Patch-Clamp Analysis

Part of the book series: Neuromethods ((NM,volume 35))

Abstract

The great advantage of patch clamp in cellular physiology is that it allows sensitive and reliable analysis of the electrical activity of cell membranes at the molecular level. Whole cell is the most popular variant of the patch-clamp technique. It is easy to obtain and it allows the use of intra- and extra-cellular recording solutions particularly devised to isolate the ionic membrane conductance of interest, or to intracellularly apply modulators or drugs. Patch clamp is therefore a very powerful technique, but the study of intracellular signaling-and the assessment of its impact on cellular electricity-requires the experimenter to perform recordings from virtually intact cells.

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Author information

Authors and Affiliations

  1. Department of Neurosurgery, University of Washington, Seattle, WA

    Raimondo D’Ambrosio

Authors
  1. Raimondo D’Ambrosio
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Editor information

Editors and Affiliations

  1. University of Saskatchewan, Saskatoon, Canada

    Alan A. Boulton , Glen B. Baker  & Wolfgang Walz ,  & 

  2. Alviva Biopharmaceuticals Inc., Saskatoon, Canada

    Alan A. Boulton

  3. University of Alberta, Edmonton, Canada

    Glen B. Baker

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© 2002 Humana Press Inc.

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D’Ambrosio, R. (2002). Perforated Patch-Clamp Technique. In: Walz, W., Boulton, A.A., Baker, G.B. (eds) Patch-Clamp Analysis. Neuromethods, vol 35. Humana Press. https://doi.org/10.1385/1-59259-276-7:195

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  • DOI: https://doi.org/10.1385/1-59259-276-7:195

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-013-7

  • Online ISBN: 978-1-59259-276-0

  • eBook Packages: Springer Protocols

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