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Perforated Patch Recording with β-escin

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Abstract

 Perforated patch recording with nystatin, amphotericin B and gramicidin can be more difficult in the hands of some investigators than others. In addition, it is difficult to introduce low molecular weight substances such as dyes into the cytoplasm in such experiments. We have determined that β-escin represents a convenient, easy-to-use alternative to less water-soluble ionophores.

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Authors and Affiliations

  1. Department of Physiology and Biophysics, University of Texas Medical Branch, Galveston, Texas, TX 77555-0641, USA e-mail: ppalade@utmb.edu Tel.: +1-409-7721746, Fax: +1-409-7723381, , , , , , US

    Jing-Song Fan & P. Palade

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  1. Jing-Song Fan
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  2. P. Palade
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Received: 25 June 1998 / Received after revision: 7 August 1998 / Accepted: 7 September 1998

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Fan, JS., Palade, P. Perforated Patch Recording with β-escin. Pflügers Arch 436, 1021–1023 (1998). https://doi.org/10.1007/PL00008086

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  • DOI: https://doi.org/10.1007/PL00008086

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