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Methylation-Sensitive Restriction Fingerprinting

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Part of the Methods in Molecular Biology™ book series (MIMB, volume 200)

Abstract

Methylation of cytosine residues is an almost ubiquitous finding of higher organisms (1). The majority of this methylation occurs at the dinucleotide CpG (where p denotes a phosphate group) (2). CpG sites are distributed throughout the genome with clusters of the sequence being found in the 5′ promoter region of housekeeping genes, in groups known as CpG islands. These short stretches of DNA have a have a C and G base composition, which in mammals and avians is estimated to be 10 times higher than in bulk DNA (3)

Keywords

Sodium Dodecyl Sulfate Chronic Myeloid Leukemia Methylation Status Tumor Genome Acrylamide Solution 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Humana Press Inc. 2002

Authors and Affiliations

  1. 1.Department of Medical BiochemistryUniversity of Wales College of MedicineCardiffUK

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