DNA Methylation Protocols

  • Ken I. Mills
  • Bernard H. Ramsahoye

Part of the Methods in Molecular Biology™ book series (MIMB, volume 200)

Table of contents

  1. Front Matter
    Pages i-x
  2. Ken I. Mills, Bernard H. Ramsahoye
    Pages 1-8
  3. Bernard H. Ramsahoye
    Pages 9-16
  4. Piroska E. Szabó, Jeffrey R. Mann, Gerd P. Pfeifer
    Pages 29-42
  5. Catherine S. Davies
    Pages 43-52
  6. Joseph F. Costello, Christoph Plass, Webster K. Cavenee
    Pages 53-70
  7. Cindy A. Eads, Peter W. Laird
    Pages 71-86
  8. Pearlly S. Yan, Susan H. Wei, Tim Hui-sing Huang
    Pages 87-100
  9. Peter L. Jones, Paul A. Wade, Alan P. Wolffe
    Pages 131-141
  10. Petra Hajkova, Osman El-Maarri, Sabine Engemann, Joachim Oswald, Alexander Olek, Jörn Walter
    Pages 143-154
  11. Moshe Szyf, Sanjoy K. Bhattacharya
    Pages 155-161
  12. Moshe Szyf, Sanjoy K. Bhattacharya
    Pages 163-176
  13. Back Matter
    Pages 177-189

About this book

Introduction

Since methylation is now understood to be integral to DNA replication and transcription, with abnormalities frequently found to occur during tumorigenesis, the number of breakthrough techniques for methylation's study has sharply increased. In DNA Methylation Protocols, an internationally well-recognized panel of investigators offer a set of readily reproducible protocols for the analysis of DNA methylation and methylases. These powerful methods provide the tools necessary for studying methylation at both the global level and the level of sequence, and include many techniques for identifying genes that might be aberrantly methylated in cancer and aging. Additional methods cover genome-wide analysis of abnormal DNA methylation, and the isolation and measurement of demethylases and related proteins. Each technique is described by a hands-on master, and includes a concise summary of the basic theory, a complete materials list, step-by-step instructions for its successful execution, and helpful notes on avoiding pitfalls.
Comprehensive and cutting-edge, DNA Methylation Protocols brings together not only the basic methods, but also the many exciting new techniques available today for the analysis of gene- and site-specific DNA methylation, and will prove invaluable to all researchers investigating replication, transcription, growth, differentiation, and carcinogenesis today.

Editors and affiliations

  • Ken I. Mills
    • 1
  • Bernard H. Ramsahoye
    • 2
  1. 1.Department of HaematologyUniversity of Wales College of MedicineCardiffUK
  2. 2.Department of HaematologyWestern General HospitalEdinburghUK

Bibliographic information

  • DOI https://doi.org/10.1385/1592591825
  • Copyright Information Humana Press 2002
  • Publisher Name Springer, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-618-5
  • Online ISBN 978-1-59259-182-4
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book