Abstract
The tear film provides a refractive optical surface of the eye and nutrients to the underlying epithelial cells; it also contains factors that protect the eye from invasion by foreign particles including microorganisms. The tear film can alter rapidly from a basal state that occurs during the daytime, to the extremes of a rapidly increased lacrimation that occurs during crying (either as the result of physical trauma or emotional trauma) or a cessation of tear flow that occurs during eye closure. During these changes the protein and lipid components of the tear film have been shown to alter dramatically. Antibacterial proteins such as lysozyme and lactoferrin are designated as regulated protein, i.e., their concentration as a function of tear volume does not alter during increased lacrimation (1). On the other hand, secretory IgA, the predominant immunoglobulin in tears, is a constitutively secreted protein, i.e., its concentration alters during changes in lacrimation (1).
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References
Fullard, R. J. and Snyder, C. (1990) Protein levels in non-stimulated and stimulated tears of normal subjects. Invest. Ophthalmol. Vis. Sci. 31, 1119ā1126.
Sack, R. A., Tan, K. O., and and Tan, A. (1992) Diurnal tear cycle: evidence for a nocturnal inflammatory constitutive tear fluid. Invest. Ophthalmol. Vis. Sci. 33, 626ā640.
Tan, K. O., Sack, R. A., Holden, B. A., and Swarbrick, H. A. (1993) Temporal sequence of changes in tear film composition during sleep. Curr. Eye Res. 12, 1001ā1007.
Thakur, A., Willcox, M. D. P., and Stapleton, F. (1998) The proinflammatory cytokines and arachidonic acid metabolites in human overnight tears: homeostatic mechanisms. J. Clin. Immunol. 18, 61ā70.
Thakur, A. and Willcox, M. (2000) Contact lens wear alters the production of certain inflammatory mediators in tears. Exp. Eye Res. 70, 1ā5.
Thakur, A. and Willcox, M. (1998) Cytokine and lipid inflammatory mediator profile of human tear during contact lens associated inflammatory diseases. Exp. Eye Res. 67, 9ā19.
Wakefield, D., McCluskey, P., Roche, N., and Rosio, J. L. (1995) Aqueous humor cytokine profile in patients with chronic uveitis. Ocular Immunol. Inflamm. 3, 203ā208.
Di Persio, J. F., Billing, P., Williams, R., and Gasson, J. C. (1988) Human granulocyte-macrophage colony stimulating factor and other cytokines prime human neutrophils for enhanced arachidonic acid release and LTB4 synthesis. J. Immunol. 140, 4315ā4322.
Wirthmueller, U. A., De Weck, L., and Dahinden, C. A. (1989) PAF production in human neutrophils by sequential stimulation with GM-CSF and the chemotactic factors C5a or fMLP. J. Immunol. 142, 3213ā3218.
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Thakur, A., Willcox, M.D.P. (2001). Detection of Cytokines in Tears. In: OāNeill, L.A.J., Bowie, A. (eds) Interleukin Protocols. Methods in Molecular Medicineā¢, vol 60. Springer, Totowa, NJ. https://doi.org/10.1385/1-59259-146-9:345
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DOI: https://doi.org/10.1385/1-59259-146-9:345
Publisher Name: Springer, Totowa, NJ
Print ISBN: 978-0-89603-738-0
Online ISBN: 978-1-59259-146-6
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