Interleukin Protocols

  • Luke A. J. O’Neill
  • Andrew Bowie

Part of the Methods in Molecular Medicine™ book series (MIMM, volume 60)

Table of contents

  1. Front Matter
    Pages i-xv
  2. General Methods for Assaying Interleukin Protein and mRNA

    1. Front Matter
      Pages 1-1
    2. Catherine Greene
      Pages 3-15
    3. Almuth Christine Hauer, Mona Bajaj-Elliott
      Pages 17-28
    4. Liam O’Mahony, Maurice O’Donoghue
      Pages 41-48
    5. Mona Bajaj-Elliott, Almuth Christine Hauer
      Pages 49-58
    6. Ellen M. Nilsen, Finn-Eirik Johansen, Per Brandtzaeg
      Pages 59-76
    7. Moshe Ligumsky, Hovav Nechushtan, Ehud Razin
      Pages 77-81
    8. Ginette Tardif, John A. Di Battista, Jean-Pierre Pelletier, Johanne Martel-Pelletier
      Pages 99-109
    9. Scott W. Van Arsdell, Kevin P. Murphy, Csaba Pazmany, Diane Erickson, Mark D. Moody
      Pages 111-124
    10. Mahmoud Huleihel
      Pages 157-162
  3. Illustration of Methodologies Using Individual Interleukins

    1. Front Matter
      Pages 175-175
    2. Paul N. Moynagh
      Pages 177-183
    3. Lyanne Weston
      Pages 185-192
    4. Amos Douvdevani, Michal Weiler, Eli Lewis
      Pages 193-204

About this book


Interleukins, a family of proteins that regulate immunity and inflammation throughout the body, are also involved in autoimmune disease (rheumatoid arthritis, multiple sclerosis, and asthma); are implicated in such other conditions as cancer, myocardial infarction, and depression; and have value in the diagnosis and prognosis of many diseases. Luke O'Neill and Andrew Bowie bring together in Interleukin Protocols a collection of standard and advanced methods for measuring these powerful agents. Their readily reproducible techniques range from the assay of interleukin protein and mRNA-using ELISA, FACS, and RT-PCR-to the study of interleukin signal transduction. Newer techniques are also covered, including the analysis of interleukin gene polymorphisms and the use of cDNA microarrays. Many of the assays are geared to specific pathologies, including breast cancer, depression, psoriasis, Grave's disease, migraine, and myocardial infarction. There are also helpful discussions of the difficulties in measuring interleukins in different biological fluids, such as sputum from asthma patients, peritoneal fluids, synovial fluid from arthritic joints, and cerbrospinal fluid from patients with meningitis. Each step-by-step method is described by a hands-on expert and includes notes on how to avoid failure.
Comprehensive and highly practical, Interleukin Protocols offers biomedical investigators a stellar collection of all the major techniques needed to analyze the role of interleukins in disease, to improve diagnosis, and to foster the rapid emergence of new and more powerful therapeutics.

Editors and affiliations

  • Luke A. J. O’Neill
    • 1
  • Andrew Bowie
    • 2
  1. 1.Department of Biochemistry and Biotechnology InstituteTrinity CollegeDublinIreland
  2. 2.Department of Pharmacology, Conway Institute of Biomolecular and Biomedical ResearchUniversity College DublinDublinIreland

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2001
  • Publisher Name Springer, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-738-0
  • Online ISBN 978-1-59259-146-6
  • Series Print ISSN 1543-1894
  • Series Online ISSN 1940-6037
  • About this book