Abstracts
Cytokines are important mediators of the persistent cycle of inflammation and repair that characterizes chronic airway diseases such as asthma, chronic obstructive pulmonary disease (COPD), and cystic fibrosis (CF). Interleukin (IL)-5 is a key mediator of the eosinophil infiltrate that characterizes asthma, whereas IL-8 is a potent chemoattractant for neutrophils in COPD and CF. IL-5 and IL-8 can be measured in airway samples obtained by the relatively noninvasive technique of sputum induction. Procedures for sputum induction have recently been standardized, and samples of airway secretions can now be obtained from most adults and children over 7 years of age. Sputum is induced via inhalation of hypertonic saline (4.5%) and assisted expectoration (1). The measurement of cytokines in induced sputum allows a direct estimation of the airway levels of these mediators in health and disease. This chapter details methods that have successfully been applied to the immunochemical assay of IL-5 and IL-8 in induced sputum.
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References
Gibson, P. G. (1998) Use of induced sputum to examine airway inflammation in childhood asthma. J. Allergy Clin. Immunol. 102, S100–101.
Shiels, C. A., Kas, J., Travassos, W., Allen, P. G., Janmey, P. A., Wohl, M. E., et al. (1996) Actin filaments mediate DNA fiber formation in chronic inflammatory airway disease. Am. J. Pathol. 148, 919–927.
Melan, M. A. (1994) Overview of cell fixation and permabilisation, in Immuno cytochemical Methods and Protocols (Javois, L. C., ed.), Humana, Totowa, NJ, pp. 55–65.
Bratthauer, G. L. (1994) Overview of antigen detection through enzymatic activity, in Immunocytochemical Methods and Protocols (Javois, L. C., ed.), Humana, Totowa, NJ, pp. 155–164.
Boenisch, T. (1989) Background, in Handbook of Immunochemical Staining Methods (Naish, S. J., et al., eds.), DAKO, CA, Carpenteria, pp. 21–23.
Foster, P. S., Hogan, P., Matthaei, K. I., and Young, I. G. (1999) The role of interleukin-5 in allergic airways disease, in Interleukin-5: From Molecule to Drug Target for Asthma (Sanderson, C.J., ed.), Marcel Dekker, NY, pp. 127–131.
Sehmi, R. and Denburg, J. A. (1999) Bone marrow in allergic disease: the role of interleukin-5 as an eosinophilopoietic factor, in Interleukin-5: From Molecule to Drug Target for Asthma (Sanderson, C. J., ed.), Marcel Dekker, NY, pp. 69–64.
Shi, H. Z., Xiao, C. Q., Zhong, D., Qin, S. M., Liu, Y., Liang, G. R., et al. (1998) Effect of inhaled interleukin-5 on airway hyperactivity and eosinophilia in asthmatics. Am. J. Respir. Crit. Care Med. 157, 204–209.
Walls, C. M., Patel, B., Harte, T. K., Zia-Amirhosseini, P., Mithron, E., Hottenstein, C. S., et al. (1999) SB-240563, An antiIL-5 monoclonal anti-body:Tolerability, activity and pharmacokinetic assessment in patients with asthma. Eur. Respir. J. 14, P1988.
Adams, D. H. and Lloyd, A. R. (1997) Chemokines: leucocyte recruitment and activation cytokines. Lancet 349, 490–495.
Nocker, R. E. T., Schoonbrood, D. F. M., van der Graaf, E. A., Hack, C. E., Lutter, R., Jansem, H. M., et al. (1996) Interleukin-8 in airway inflammation in patients with asthma and chronic obstructive pulmonary disease. Int. Arch. Allergy Immunol. 109, 183–191.
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Gibson, P.G., Simpson, J.L. (2001). Measurement of Cytokines in Induced Sputum. In: O’Neill, L.A.J., Bowie, A. (eds) Interleukin Protocols. Methods in Molecular Medicine™, vol 60. Springer, Totowa, NJ. https://doi.org/10.1385/1-59259-146-9:305
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DOI: https://doi.org/10.1385/1-59259-146-9:305
Publisher Name: Springer, Totowa, NJ
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