Abstract
In elucidating a novel cell-cycle pathway or mode of regulation, it will first be necessary to establish that the cell is arrested in a particular stage of the cell cycle following arrest-inducing conditions. This is achieved by staining the DNA in a population of cells with propidium iodide and then determining the cell-cycle profile of that population by fluorescence-activated cell sorter (FACS) or by measuring 3H-thymidine incorporation into DNA as it is being replicated.
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Gitig, D.M., Koff, A. (2000). CDK Pathway: Cyclin-Dependent Kinases (cdks) and Cyclin-Dependent Kinase Inhibitors . In: Howe, P.H. (eds) Transforming Growth Factor-Beta Protocols. Methods in Molecular Biology™, vol 142. Humana Press. https://doi.org/10.1385/1-59259-053-5:109
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DOI: https://doi.org/10.1385/1-59259-053-5:109
Publisher Name: Humana Press
Print ISBN: 978-0-89603-646-8
Online ISBN: 978-1-59259-053-7
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