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Transforming Growth Factor-Beta Protocols

  • Philip H. Howe

Part of the Methods in Molecular Biology™ book series (MIMB, volume 142)

Table of contents

  1. Front Matter
    Pages i-x
  2. Roberta Mazzieri, John S. Munger, Daniel B. Rifkin
    Pages 13-27
  3. Andrew H. Limper
    Pages 39-54
  4. Barbara A. Hocevar, Philip H. Howe
    Pages 55-65
  5. Arun Mehra, Liliana Attisano, Jeffrey L. Wrana
    Pages 67-78
  6. Barbara A. Hocevar, Philip H. Howe
    Pages 97-108
  7. Jianbo Yue, Kathleen M. Mulder
    Pages 125-131
  8. Lois L. Myeroff, Sanford Markowitz
    Pages 133-137
  9. Lois L. Myeroff, Hongmei He, Stephen P. Fink, Sanford Markowitz
    Pages 139-147
  10. Thomas L. Brown, Supriya Patil, Philip H. Howe
    Pages 149-167
  11. Back Matter
    Pages 169-175

About this book

Introduction

The recent isolation and identification of the TGFß receptors, as well as their intracellular mediating proteins, has driven the development of many new methodologies and techniques for the study of TGFß. In Transforming Growth Factor-Beta Protocols, Philip H. Howe and a group of well-versed experimentalists present the first major collection of indispensable classic and cutting-edge TGFß assays. Described in great detail to ensure robust and successful results, these readily reproducible techniques range from the growth inhibition assay for TGFß to methodologies for monitoring its interactions with the mediating proteins. Extensive notes discuss potential pitfalls and provide tips on how to avoid failures, and throughout, emphasis is given to detailing those technical steps critical for experimental success that are often omitted in the primary literature.
Concise and highly practical, Transforming Growth Factor-Beta Protocols provides today's molecular and cell biologists-both expert and novice-with time-tested methods for the identification and analysis of the signal transduction pathways by which TGFß induces and modulates physiological behavior.

Editors and affiliations

  • Philip H. Howe
    • 1
  1. 1.The Cleveland Clinic FoundationClevelandOH

Bibliographic information

  • DOI https://doi.org/10.1385/1592590535
  • Copyright Information Humana Press 2000
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-646-8
  • Online ISBN 978-1-59259-053-7
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site