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Expression of Recombinant Matrix Metalloproteinases in Yeast

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Matrix Metalloproteinase Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 151))

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Abstract

Deciding what system to use for recombinant matrix metalloproteinase (MMP) expression is often a matter of practicality (i.e., simplicity, cost effectiveness, time considerations). Of course, the final judgment of practicality is determined by the production of sufficient quantities of functional MMPs. A system that combines the benefits of bacterial and cultured cell systems, without their disadvantages, would be of great use.

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Author information

Authors and Affiliations

  1. McArdle Laboratories for Cancer Research, University of Wisconsin-Madison, Madison, WI

    Glenn A. Doyle

Authors
  1. Glenn A. Doyle
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Editor information

Editors and Affiliations

  1. University of East Anglia, Norwich, UK

    Ian M. Clark PhD

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© 2001 Humana Press Inc.

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Doyle, G.A. (2001). Expression of Recombinant Matrix Metalloproteinases in Yeast. In: Clark, I.M. (eds) Matrix Metalloproteinase Protocols. Methods in Molecular Biology™, vol 151. Humana Press. https://doi.org/10.1385/1-59259-046-2:219

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  • DOI: https://doi.org/10.1385/1-59259-046-2:219

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-733-5

  • Online ISBN: 978-1-59259-046-9

  • eBook Packages: Springer Protocols

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