Matrix Metalloproteinase Protocols

  • Ian M. Clark

Part of the Methods in Molecular Biology™ book series (MIMB, volume 151)

Table of contents

  1. Front Matter
    Pages i-xv
  2. MMPs and TIMPs—An Overview of the Field

    1. Front Matter
      Pages 1-179
    2. J. Frederick Woessner Jr.
      Pages 1-23
    3. Gloria Velasco, Carlos López-Otín
      Pages 25-44
    4. Lisa M. Coussens, Steven D. Shapiro, Paul D. Soloway, Zena Werb
      Pages 149-179
  3. Expression and Purification of MMPs and TIMPs

    1. Front Matter
      Pages 181-304
    2. Karen M.-L. Yeow, Blaine W. Phillips, P. Paul Beaudry, Kevin J. Leco, Gillian Murphy, Dylan R. Edwards
      Pages 181-189
    3. L. Jack Windsor, Darin L. Steele
      Pages 191-205
    4. Michael J. Butler, Marie-Pia d’Ortho, Susan J. Atkinson
      Pages 239-255
    5. Ken-ichi Shimokawa, Hideaki Nagase
      Pages 275-304
  4. Detection of MMPs and TIMPs

    1. Front Matter
      Pages 305-375
    2. Howard Wong, Huong Muzik, Lori L. Groft, Marc A. Lafleur, Charles Matouk, Peter A. Forsyth et al.
      Pages 305-320

About this book

Introduction

From the simple discovery in 1962 that resorbing tadpole tail expressed an enzyme (MMP) that could degrade collagen gels, matrix metalloproteinase (MMP) research has advanced to discover more than twenty distinct vertebrate MMPs and four specific inhibitors (TIMPS), a veritable family of enzymes involved in many physiological and pathological processes. In Matrix Metalloproteinase Protocols, leading experts detail proven laboratory techniques for the study of MMPs. The methods include those for the expression and purification of MMPs and TIMPs, for the detection of MMPs and TIMPs at both the protein and mRNA levels, and for the assay of MMP and TIMP activities in a wide variety of circumstances. Each method includes step-by-step instructions and notes on variant applications and pitfalls to avoid. A selective overview of the MMP arena spells out where the field has been, where it is, and where it is going.
Comprehensive and highly practical, Matrix Metalloproteinase Protocols brings together the long and hard-earned experience of master experimentalists that will allow not only novices to get up to speed quickly, but also add to the repertoire of successful techniques in expert laboratories.

Editors and affiliations

  • Ian M. Clark
    • 1
  1. 1.University of East AngliaNorwichUK

Bibliographic information

  • DOI https://doi.org/10.1385/1592590462
  • Copyright Information Humana Press 2001
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-733-5
  • Online ISBN 978-1-59259-046-9
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book