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Autoradiography of Enzymes, Second Messenger Systems, and Ion Channels

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Receptor Binding Techniques

Part of the book series: Methods in Molecular Biology ((MIMB,volume 106))

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Abstract

Autoradiographic detection of ligand binding to tissue sections has been used to localize, quantify, and characterize a diverse range of sites. Enzymes have been studied using selective inhibitors, ion channels using naturally occurring toxins, and second messenger systems using inositol polyphosphates. Ligand binding complements immunohistochemistry and in situ hybridization by permitting pharmacological characterization and quantification of active sites. Localization, affinity, and specificity of binding sites for ligands can be correlated with functional studies performed with the same pharmacological agent. Bioactive ligands are often identified before their targets have been fully characterized, and radiolabeled ligands may become available before molecular and immunological reagents have been developed. A pharmacologically active agent may be synthesized before the endogenous ligand for its binding site has been identified, and autoradiographic methods may help elucidate the site of action of such agents.

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Author information

Authors and Affiliations

  1. Rheumatology Unit, University of Nottingham, City Hospital, Nottingham, UK

    David A. Walsh

  2. Department of Histochemistry, Imperial College of Science and Technology, Hamrnersmith Hospital, London, UK

    John Wharton

Authors
  1. David A. Walsh
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  2. John Wharton
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Editor information

Editors and Affiliations

  1. The Medical School, University of Birmingham, Birmingham, UK

    Mary Keen

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© 1999 Humana Press Inc., Totowa, NJ

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Walsh, D.A., Wharton, J. (1999). Autoradiography of Enzymes, Second Messenger Systems, and Ion Channels. In: Keen, M. (eds) Receptor Binding Techniques. Methods in Molecular Biology, vol 106. Springer, Totowa, NJ. https://doi.org/10.1385/0-89603-530-1:199

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  • DOI: https://doi.org/10.1385/0-89603-530-1:199

  • Publisher Name: Springer, Totowa, NJ

  • Print ISBN: 978-0-89603-530-0

  • Online ISBN: 978-1-59259-579-2

  • eBook Packages: Springer Protocols

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