Receptor Binding Techniques

  • Mary Keen

Part of the Methods in Molecular Biology book series (MIMB, volume 106)

Table of contents

  1. Front Matter
    Pages i-x
  2. Introduction

    1. Front Matter
      Pages 1-1
  3. Radioligand Binding in Different Preparations

    1. Front Matter
      Pages 25-25
    2. Alan L. Hudson, Lisa A. Lione
      Pages 37-47
    3. Stephanie Fletcher, Nicholas M. Barnes
      Pages 49-71
    4. Jennifer A. Koenig
      Pages 89-98
    5. John Wharton, David A. Walsh
      Pages 99-118
  4. General Considerations

    1. Front Matter
      Pages 37-37
    2. Edward C. Hulme
      Pages 139-185
  5. The Use of Binding to Study Nonreceptor Sites

    1. Front Matter
      Pages 197-197
    2. Michelle Qume, Jacqueline K. Miller
      Pages 215-229
    3. Rachel M. C. Parker, Nicholas M. Barnes
      Pages 247-283
  6. Back Matter
    Pages 285-290

About this book

Introduction

In Receptor Binding Techniques, detailed experimental protocols devised by expert practitioners demonstrate the power of radioligand binding for studying a wide variety of receptors. The methods can be applied to G protein-coupled receptors, receptors with integral ion channels, and sites other than receptors, such as guanine nucleotide binding proteins, enzymes, transporters, and mRNA. The individual protocols encompass many different preparations, ranging from solubilized receptors, through membrane preparations, intact cells, and autoradiography in tissue slices, to PET and SPECT analysis of binding in living human brain. Along with the detailed, easily reproducible instructions, the protocols also take into account the problems inherent in the basic binding technique, such as common experimental artifacts and the definition of receptor sites, as well as problems associated with the use of particular ligands and receptor preparations.

Receptor Binding Techniques shows clearly how these techniques can be adapted to many different applications and fully utilized in drug discovery programs. It will be of high value not only to researchers new to the field of receptor binding, but also to more experienced workers, serving as an authoritative bench manual replete with optimalized practical methods and protocols.

Pharmacologists, neuroscientists, cell biologists, molecular biologists, and biochemists

Editors and affiliations

  • Mary Keen
    • 1
  1. 1.The Medical SchoolUniversity of BirminghamBirminghamUK

Bibliographic information

  • DOI https://doi.org/10.1385/0896035301
  • Copyright Information Humana Press 1999
  • Publisher Name Springer, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-530-0
  • Online ISBN 978-1-59259-579-2
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book