Abstract
Immunoelectron microscopy (IEM) techniques, usually in conjunction with light microscopy techniques, are used for the localization of antigenic molecules that may be employed for vaccine development, organelle characterization, or other biologically relevant applications. Typically, the steps involved in IEM include fixation, dehydration and embedding, cutting and mounting, immunolabeling, observation, and documentation.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Griffiths G. (ed.) (1993) Fine Structure Immunocytochemistry. Springer-Verlag, Berlin.
Aikawa, M. and Atkinson, C.T. (1990) Immunoelectron microscopy of parasites. Adv. Parasitol. 29, 151–214.
Beesley, J. E. (1986) The use of gold markers in immunocytochemical studies of microbiological organisms: a review. J. Microsc. 143, 177–186.
Suarez, C. E., Palmer, G. H., Hines, S. A., and McElwain, T. F. (1993) Immunogenic B-cell epitopes of Babesia bovis rhoptry-associated protein 1 are distinct from sequences conserved between species. Infect. Immun. 61, 3511–3517.
Fahmi, A. (1967) An extemporaneous lead citrate stain for electron microscopy. Proc. Annu. Meet. Electron Microsc. Soc. Am. 25, 148.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1995 Humana Press Inc.
About this protocol
Cite this protocol
Suarez, C.E., Brown, R. (1995). Use of Monoclonal Antibodies in Immunoelectron Microscopy. In: Davis, W.C. (eds) Monoclonal Antibody Protocols. Methods in Molecular Biology™, vol 45. Humana Press. https://doi.org/10.1385/0-89603-308-2:109
Download citation
DOI: https://doi.org/10.1385/0-89603-308-2:109
Publisher Name: Humana Press
Print ISBN: 978-0-89603-308-5
Online ISBN: 978-1-59259-532-7
eBook Packages: Springer Protocols