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Monoclonal Antibody Protocols

  • William C. Davis

Part of the Methods in Molecular Biology™ book series (MIMB, volume 45)

Table of contents

  1. Front Matter
    Pages i-x
  2. Mary J. Hamilton, William C. Davis
    Pages 17-28
  3. Brenda V. Jones, Christopher J. Howard
    Pages 41-47
  4. Araceli L. Lumanglas, Bosco Shang Wang
    Pages 49-54
  5. Lance E. Perryman, Patricia H. Mason
    Pages 55-60
  6. Philippe Pouletty
    Pages 69-78
  7. Jon A. Rudbach, John L. Cantrell, J. T. Ulrich
    Pages 79-82
  8. Morris D. Cooper, Risa Kirkpatrick
    Pages 83-87
  9. Shan-Rong Shi, Jeffrey B. Prince, Christopher M. Jones, Krishan L. Kalra, Atul K. Tandon
    Pages 89-108
  10. Carlos E. Suarez, Ruth Brown
    Pages 109-114
  11. Carleton C. Stewart, Sigrid J. Stewart
    Pages 129-147
  12. Uwe Marx, Wolfram Merz
    Pages 169-176
  13. Rosaria P. Haugland
    Pages 205-221
  14. Rosaria P. Haugland, Wendy W. You
    Pages 223-233
  15. Rosaria P. Haugland
    Pages 235-243
  16. Back Matter
    Pages 261-264

About this book

Introduction

Since the initial description of techniques to immortalize anti­ body-producing B-lymphocytes by fusion with tissue culture-adapted myeloma cells, methods have been developed to produce monoclonal antibodies of defined specificity in multiple animal species. Stable hybrids can be readily produced in mice using a number of myeloma and hybridoma cell lines. To obviate the problem of identifying fusion partners in other animal species, xenohybrids have been produced using B-lymphocytes from the relevant species and mouse myeloma cells. The use of xenohybrids has minimized the problem of obtain­ ing stable antibody-producing hybrids in all species examined thus far. Although alternative techniques are being developed to produce monoclonal antibodies by molecular methods, hybridoma technol­ ogy will remain the technology of choice for producing monoclonal antibodies for a variety of applications in research and industry. The objective of Monoclonal Antibody Protocols is to provide investigators with a set of methods for producing and using mono­ clonal antibodies in biomedical, agricultural, and biological sciences. The book is not intended to provide methodology for all possible applications, but rather a series of methods presented in an easy-- follow format that can be used by new and established investiga­ tors, graduate and postgraduate fellows, and technical staff.

Editors and affiliations

  • William C. Davis
    • 1
  1. 1.Department of Veterinary Microbiology and Pathology, College of Veterinary MedicineWashington State UniversityPullman

Bibliographic information

  • DOI https://doi.org/10.1385/0896033082
  • Copyright Information Humana Press 1995
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-308-5
  • Online ISBN 978-1-59259-532-7
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site