Abstract
The Sinuclease protection procedure allows the precise definition of the beginning and end of gene transcripts as well as the position of intron/ exon boundaries in a gene. Alternatively, when these parameters are already known, the technique can be used to quantify transcript levels in a variety of expression systems.
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© 1991 The Humana Press Inc., Clifton, NJ
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Walmsley, M.E., Patient, R.K. (1991). Quantitative and Qualitative Analysis of Exogenous Gene Expression by the S1 Nuclease ProtectionAssay. In: Murray, E.J. (eds) Gene Transfer and Expression Protocols. Methods in Molecular Biology, vol 7. Humana Press. https://doi.org/10.1385/0-89603-178-0:271
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DOI: https://doi.org/10.1385/0-89603-178-0:271
Publisher Name: Humana Press
Print ISBN: 978-0-89603-178-4
Online ISBN: 978-1-59259-494-8
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