Gene Transfer and Expression Protocols

  • E. J. Murray

Part of the Methods in Molecular Biology book series (MIMB, volume 7)

Table of contents

  1. Front Matter
    Pages i-xiii
  2. Rémy Aubin, Michael Weinfeld, Malcolm C. Paterson
    Pages 3-13
  3. Remy Aubin, Michael Weinfeld, Malcolm C. Paterson
    Pages 35-43
  4. Sarah C. Spencer
    Pages 45-52
  5. Peter N. Goodfellow
    Pages 53-61
  6. Janet M. Shipley, Denise Sheer
    Pages 99-106
  7. Frank L. Graham, Ludvik Prevec
    Pages 109-128
  8. Michael Mackett
    Pages 129-146
  9. Murk J. Bailey, Robert D. Possee
    Pages 147-168
  10. Marie-Louise Hammurskjöld
    Pages 169-180
  11. Jay P. Morgenstern, Hartmut Land
    Pages 181-206
  12. Nicholas C. Wrighton
    Pages 209-215
  13. Grant R. MacGregor, Gamy P. Nolan, Steven Fiering, Mario Roederer, Leonard A. Herzenberg
    Pages 217-235
  14. Robert F. Santerre, Jenna D. Walls, Brian W. Grinnell
    Pages 245-256

About this book

Introduction

Biology is the study of living things. The classical approach might be described as holistic and descriptive, whereas the modern molecular - proach aims to be investigative, reductionist, and mechanistic . Genes contain all the information for the structure of all living things ; thus, the understanding of how genes are regulated is an important step toward understanding the nature of living things. The study of gene regulation has been made more tractable by the design of simple expe- mental models in which a single gene can be isolated from the milieu of the organism. The new science of molecular biology has introduced techniques that permit the design of such experimental models. In - sence, the genome of the organism is dissected in such a manner that specific genes may now be introduced into an appropriate cell line . Subsequent analysis of the proteins expressed from the genes under study results in the identification of the regulatory DNA sequences .

Editors and affiliations

  • E. J. Murray
    • 1
  1. 1.Roche Products Ltd.Welwyn Garden CityUK

Bibliographic information

  • DOI https://doi.org/10.1385/0896031780
  • Copyright Information Humana Press 1991
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-178-4
  • Online ISBN 978-1-59259-494-8
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book