Epidermis-Targeted Gene Transfer Using In Vivo Electroporation

  • Hiroki Maruyama
  • Jun-Ichi Miyazaki
  • Fumitake Gejyo
Part of the Methods in Molecular Biology™ book series (MIMB, volume 289)


The skin is an important target for gene transfer because of its easy accessibility. Recently, we demonstrated that an in vivo electroporation approach could be applied to plasmid deoxyribonucleic acid delivery in rat skin of the abdominal area. Plate-and-fork type electrodes were effective for gene delivery by skin-targeted gene transfer. Eight 12–24 V, 50-m electric pulses were delivered through the electrodes. Skin injection with pCAGGS-lacZ showed lacZ gene expression in the upper most cell layers (horny, granular, and prickle cell layers) of the epidermis on day 1 and in the subcutaneous muscle layer on day 7 after the pCAGGS-lacZ transfer. Slight skin damage as the result of the gene transfer procedure was evident on day 1 but absent by day 7. These results demonstrate that plasmid deoxyribonucleic acid transfer by in vivo electroporation at low voltage is a useful procedure for short-term skin-targeted gene transfer.

Key Words

Electroporation gene transfer skin plasmid DNA epidermis electrodes CAG promoter 


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Copyright information

© Humana Press Inc. 2005

Authors and Affiliations

  • Hiroki Maruyama
    • 1
  • Jun-Ichi Miyazaki
    • 2
  • Fumitake Gejyo
    • 1
  1. 1.Division of Clinical Nephrology and RheumatologyNiigata University Graduate School of Medical and Dental SciencesNiigataJapan
  2. 2.Division of Stem Cell Regulation ResearchOsaka University Medical SchoolOsakaJapan

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