Epidermal Cells

Methods and Protocols

  • Kursad Turksen

Part of the Methods in Molecular Biology™ book series (MIMB, volume 289)

Table of contents

  1. Front Matter
    Pages i-xv
  2. Keratinocyte and Organ Cultures

    1. Front Matter
      Pages 1-1
    2. Annalisa Pirrone, Barbara Hager, Philip Fleckman
      Pages 3-14
    3. Richard P. Redvers, Pritinder Kaur
      Pages 15-22
    4. Monika Jost, Ulrich Rodeck
      Pages 23-28
    5. Nicole Maas-Szabowski, Norbert E. Fusenig, Hans-Jürgen Stark
      Pages 47-60
    6. Alexander Margulis, Weitian Zhang, Jonathan A. Garlick
      Pages 61-70
  3. Epidermal Stem Cells

    1. Front Matter
      Pages 71-71
    2. Wei-Yang Wu, Rebecca J. Morris
      Pages 73-77
    3. Amy Li, Pritinder Kaur
      Pages 87-96
    4. Danielle Larouche, Cindy Hayward, Kristine Cuffley, Lucie Germain
      Pages 103-110
  4. Analysis of Epidermal Differentiation

    1. Front Matter
      Pages 111-111
    2. Tammy-Claire Troy, Ramtin Rahbar, Bilge Diker, Kursad Turksen
      Pages 113-119
    3. Tammy-Claire Troy, Robert Man-Kit Cheung, Kursad Turksen
      Pages 121-126
    4. Ashley E. Webb, David Kimelman
      Pages 137-145

About this book

Introduction

In recent years our ability to understand and manipulate epidermal cells has increased tremendously, opening significant new possibilities in both basic science research and in regenerative medicine, including wound healing and transplantation. In Epidermal Cells: Methods and Protocols, a panel of experienced basic and clinical researchers describe in step-by-step detail the powerful methods they have developed and optimized to analyze and manipulate epidermal cell precursors and mature epidermal cells. These protocols cover different methods and models for culturing epidermal cells, for enriching very early epidermal progenitors, as well as for studying epidermal cell commitment and differentiation both in vitro and in vivo. Topics of special interest include the derivation, characterization, and utility of epidermal stem cells, mature epidermal cells and their characterization, and applications in regenerative medicine. The protocols follow the successful Methods in Molecular Biology™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
State-of-the-art and highly practical, Epidermal Cells: Methods and Protocols offers experienced and novice investigators alike an invaluable collection of readily reproducible techniques designed to broaden not only our understanding of the biology of epidermal cells, but also their utility in normal tissue homeostasis and regenerative medicine applications.

Keywords

DNA PAC PCR Promoter gene expression genes reproducible techniques

Editors and affiliations

  • Kursad Turksen
    • 1
  1. 1.Ottawa Health Research InstituteOttawaCanada

Bibliographic information

  • DOI https://doi.org/10.1385/1592598307
  • Copyright Information Humana Press 2005
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-267-4
  • Online ISBN 978-1-59259-830-4
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book