Abstract
Culture of isolated microglia from dissociated cortical tissue has promoted the in vitro study of microglial function and morphological characteristics (Giulian and Baker, 1986; Streit and Kincaid-Colton, 1995). However, cultures prepared in this manner demonstrate characteristic “ameboid” morphology, and are generally considered to be more activated than the normal, resting microglia found in situ (Sedgwick et al., 1991). To reduce this problem, as well as to recreate a cellular architecture more typical of in vivo conditions, we have utilized an organotypic tissue slice method for culture of microglia (Czapiga and Colton, 1999; Gahwiler et al., 1997). This technique allows visualization of microglial morphology and the determination of certain microglial functional parameters, in a culture environment more reminiscent of the in vivo brain.
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Further Reading
Behar, T., Scott, C., Greene, C., Wen, X-L, Smith, S., Marie, D., Liu, Q., Barker, I., and Colton, C. (1999), Glutamate acting at NMDA receptors stimulates embryonic cortical neuronal migration. J. Neurosci. 19, 4449–4461.
Colton, C., Abel, C., Patchett, J., Keri, J., and Yao, J. (1992), Lectin staining of cultured CNS microglia. J. Histochem. Cytochem. 40, 505–512.
Czapiga, M. and Colton, C. (1999), Function of microglia in organotypic slice cultures. J. Neurosci. Res. 56, 644–651.
Gähwiler, B. H., Capogna, M., Debanne, D., McKinney, R. A., and Thompson, S. M. (1997), Organotypic slice cultures: a technique has come of age. Trends Neurosci. 20, 471–477.
Giulian, D. and Baker, T. J. (1986), Characterization of ameboid microglia isolated from the developing mammalian brain. J. Neurosci. 6, 2163–2178.
Hailer, N. P., Jarhult, J. D., and Nitsch, R. (1996), Resting microglial cells in vitro: analysis of morphology and adhesion molecule expression in organotypic hippocampal slice cultures. Glia 18, 319–331.
Jones, E. G. and Senft, J. A. (1985), An improved method to determine cell viability by simultaneous staining with fluorescein diacetate-propidium iodide. J. Histochem. Cytochem. 33, 77–79.
Murabe, Y. and Sano, Y. (1983), Morphological studies on neuroglia. VII. Distribution of “brain macrophages” in brains of neonatal and adult rats, as determined by means of immunohistochemistry. Cell Tissue Res. 229, 85–95.
Perry, V. H. and Gordon, S. (1991), Macrophages and the nervous system. Int. Rev. Cytol. 125, 203–244.
Rio-Hortega, P. (1932), in: Cytology and Cellular Pathology of the Nervous System, Penfield, W., eds. Hocker, New Yori, pp. 481–584.
Sedgwick, J. D., Schwender, S., Imrich, H., Dörries, R., Butcher, G. W., and Mulen, V. T. (1991), Isolation and direct characterization of resident microglial cells from the normal and inflamed central nervous system. Proc. Natl. Acad. Sci. USA 88, 7438–7442.
Streit, W. J. and Kincaid-Colton, C. A. (1995), The brain’s immune system. Sci. Am. 273, 38–43.
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© 2001 Humana Press Inc., Totowa, NJ
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Colton, C.A., Czapiga, M., Behar, T.N. (2001). Slice Cultures for Study of Microglia. In: Fedoroff, S., Richardson, A. (eds) Protocols for Neural Cell Culture. Springer Protocols Handbooks. Humana Press. https://doi.org/10.1385/1-59259-207-4:29
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DOI: https://doi.org/10.1385/1-59259-207-4:29
Publisher Name: Humana Press
Print ISBN: 978-0-89603-902-5
Online ISBN: 978-1-59259-207-4
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