Protocols for Neural Cell Culture

  • Sergey Fedoroff
  • Arleen Richardson

Table of contents

  1. Front Matter
    Pages i-xxii
  2. Valérie Castellani, Jürgen Bolz
    Pages 1-12
  3. Dominique Muller, Nicolas Toni, Pierre-Alain Buchs, Lorena Parisi, Luc Stoppini
    Pages 13-27
  4. Carol A. Colton, Meggan Czapiga, Toby N. Behar
    Pages 29-37
  5. Bernard Rogister, Gustave Moonen
    Pages 39-48
  6. Bernhard H. J. Juurlink
    Pages 59-69
  7. Mary I. Johnson
    Pages 71-94
  8. Mary I. Johnson, Richard P. Bunge, Patrick M. Wood
    Pages 95-115
  9. V. Wee Yong, Jack P. Antel
    Pages 129-138
  10. Bernhard H. J. Juurlink, Shawn K. Thorburne, Richard M. Devon
    Pages 149-160
  11. Ronald Doucette
    Pages 161-171
  12. Angela Gritti, Rossella Galli, Angelo Luigi Vescovi
    Pages 173-197
  13. Paul Honegger, Florianne Monnet-Tschudi
    Pages 199-218
  14. Kaoru Murayama, Natalia N. Singh, Angela Helmrich, David W. Barnes
    Pages 219-228
  15. Sonia N. Yeung, Frank Tufaro
    Pages 229-244
  16. Paul J. Price, Gregory J. Brewer
    Pages 255-264

About this book

Introduction

In Protocols for Neural Cell Culture, Third Ed., Sergey Fedoroff and Arleen Richardson extensively revise, update, and expand their best-selling and highly praised collection of readily reproducible neural tissue culture protocols. This 3rd edition adds 11 chapters describing important new procedures for the isolation, growth, and characterization of neural stem cells and for the manipulation of glial progenitor cells, as well as essential procedures for hippocampal and microglial slice cultures and transfection of neurons in culture with adenovirus. It includes key techniques for the preparation of substrata, the use of serum-free media, maintaining hybridomas, and the production and purification of monoclonal antibodies. For scientists not trained in neuroanatomy, but faced with dissecting the brain and spinal cord, most chapters in the 3rd edition provide fully detailed dissection procedures.
Protocols for Neural Cell Culture, Third Ed. is a richly augmented updating of the tried and tested laboratory procedures that have made earlier editions an indispensable reference and guide to neural cell culture. Its unique wealth of practical detail on a wide range of tissue culture systems having many applications ensure that this new edition will remain an essential resource for all investigators using cell culture methodology in studying the brain and its disorders.

Keywords

brain central nervous system myelin nervous system neurons neuroscience

Editors and affiliations

  • Sergey Fedoroff
    • 1
  • Arleen Richardson
    • 1
  1. 1.Department of Anatomy and Cell Biology, School of MedicineUniversity of SaskatchewanSaskatoonCanada

Bibliographic information

  • DOI https://doi.org/10.1385/1592592074
  • Copyright Information Humana Press 2001
  • Publisher Name Humana Press
  • eBook Packages Springer Book Archive
  • Print ISBN 978-0-89603-902-5
  • Online ISBN 978-1-59259-207-4
  • About this book