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Microinjection Manipulations in the Elucidation of Xenopus Brain Development

  • Cristine Smoczer
  • Lara Hooker
  • Saqib S. Sachani
  • Michael J. Crawford
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1082)

Abstract

Microinjection has a long and distinguished history in Xenopus and has been used to introduce a surprisingly diverse array of agents into embryos by both intra- and intercellular means. In addition to nuclei, investigators have variously injected peptides, antibodies, biologically active chemicals, lineage markers, mRNA, DNA, morpholinos, and enzymes. While enumerating many of the different microinjection approaches that can be taken, we will focus upon the mechanical operations and options available to introduce mRNA, DNA, and morpholinos intracellularly into early stage embryos for the study of neurogenesis.

Key words

Microinjection Morpholino mRNA Lineage marker Xenopus Over-expression Knockdown Transgenic Mutant 

Notes

Acknowledgment

M.J.C. is supported by the Natural Sciences and Engineering Research Council (NSERC) of Canada Grant #203549.

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Copyright information

© Springer Science+Business Media, LLC 2014

Authors and Affiliations

  • Cristine Smoczer
    • 1
  • Lara Hooker
    • 1
  • Saqib S. Sachani
    • 1
  • Michael J. Crawford
    • 1
  1. 1.Biological SciencesUniversity of WindsorWindsorCanada

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