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Proteotyping of Holm Oak (Quercus ilex subsp. ballota) Provenances Through Proteomic Analysis of Acorn Flour

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Plant Proteomics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1072))

Abstract

Proteomics has become a powerful tool to characterize biodiversity and natural variability in plant species, as well as to catalogue and establish phylogenetic relationships and distances among populations, provenances or ecotypes. In this chapter, we describe the standard proteomics workflow that we currently use in cataloguing Holm oak (Quercus ilex subsp. ballota [Desf.] Samp.) populations. Proteins are extracted from acorn flour or pollen by TCA/acetone or TCA/acetone-phenol methods, resolved by one- or two-dimensional gel electrophoresis, and gel images are captured and analyzed by appropriate software and statistical packages. Quantitative or qualitative variable bands or spots are subjected to MS analysis in order to identify them and correlate differences in the protein profile with the phenotypes or environmental conditions.

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Acknowledgments

Jose Valero was recipient of an Alban Program fellowship (I06D00010MX). This work was supported by the Spanish Ministry of Science and Innovation cofinanced by the European Community FEDER funds: CGL2008-04503-C03-01/BOS, AGL2002-00530, and AGL2009-12243-C02-02; the Regional Government of Andalusia (Junta de Andalucía; the University of Córdoba (AGR-0164: Agricultural and Plant Biochemistry and Proteomics Research Group)); and the Autonomous University of Juarez City.

Author information

Authors and Affiliations

  1. Department of Chemistry-Biology, Biomedical Sciences Institute, Autonomous University of Ciudad Juárez, Chihuahua, México

    José Valero Galván

  2. Agroforestry and Plant Biochemistry and Proteomics Research Group, Department of Biochemistry and Molecular Biology, University of Córdoba, Agrifood Campus of International Excellence, Córdoba, Spain

    Raquel González Fernández

  3. Department of Molecular Systems Biology, University of Vienna, Vienna, Austria

    Luis Valledor

  4. Department of Forestry Engineering, ETSIAM, University of Córdoba, Agrifood Campus of International Excellence, ceiA3, Córdoba, Spain

    Rafael Ma. Navarro Cerrillo

  5. Agroforestry and Plant Biochemistry and Proteomics Research Group, Department of Biochemistry and Molecular Biology, University of Córdoba, Agrifood Campus of International Excellence, ceiA3, Córdoba, Spain

    Jesus V. Jorrin-Novo

Authors
  1. José Valero Galván
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  2. Raquel González Fernández
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  3. Luis Valledor
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  4. Rafael Ma. Navarro Cerrillo
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  5. Jesus V. Jorrin-Novo
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Editor information

Editors and Affiliations

  1. Dept of Biochem & Molecular Bio, University of Cordoba, Cordoba, Spain

    Jesus V. Jorrin-Novo

  2. University of Tsukuba National Institute of Crop Science, Tsukuba, Japan

    Setsuko Komatsu

  3. Molecular Systems Biology, University of Vienna, Vienna, Austria

    Wolfram Weckwerth

  4. Dept. Molecular Systems Biology, University of Vienna, Vienna, Austria

    Stefanie Wienkoop

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Galván, J.V., Fernández, R.G., Valledor, L., Cerrillo, R.M.N., Jorrin-Novo, J.V. (2014). Proteotyping of Holm Oak (Quercus ilex subsp. ballota) Provenances Through Proteomic Analysis of Acorn Flour. In: Jorrin-Novo, J., Komatsu, S., Weckwerth, W., Wienkoop, S. (eds) Plant Proteomics. Methods in Molecular Biology, vol 1072. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-631-3_49

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  • DOI: https://doi.org/10.1007/978-1-62703-631-3_49

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-630-6

  • Online ISBN: 978-1-62703-631-3

  • eBook Packages: Springer Protocols

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