Abstract
UV/Vis spectroscopy is a simple and convenient method to determine protein concentrations as well as the average number of drugs that are conjugated to the antibody in an antibody–drug conjugate (ADC). Using the measured absorbances of the ADC and the extinction coefficients of the antibody and the drug, the average drug-to-antibody ratio (DAR) can be determined.
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Acknowledgements
We thank Pat Rancatore, Richard Seipert, Boyan Zhang, and Andrea Ji for comments and help in preparing this review.
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Chen, Y. (2013). Drug-to-Antibody Ratio (DAR) by UV/Vis Spectroscopy. In: Ducry, L. (eds) Antibody-Drug Conjugates. Methods in Molecular Biology, vol 1045. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-541-5_16
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DOI: https://doi.org/10.1007/978-1-62703-541-5_16
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